9THH
OmRV WT infectious clone
Summary for 9THH
| Entry DOI | 10.2210/pdb9thh/pdb |
| EMDB information | 55927 |
| Descriptor | Omono River Virus AK4 strain Subunit A (1 entity in total) |
| Functional Keywords | omono river virus, artiviruses, dsrna viruses, capsid, infectious clone, virus |
| Biological source | Omono River virus |
| Total number of polymer chains | 2 |
| Total formula weight | 367432.09 |
| Authors | |
| Primary citation | Garcia Hernandez, G.,Manesco, C.,Wang, H.,Okamoto, K. Crown protein is dispensable for OmRV entry but required for efficient transmission. Virology, 621:110922-110922, 2026 Cited by PubMed Abstract: Non-enveloped icosahedral dsRNA viruses, such as taxons of the Totiviridae family, have evolved surface features that enable infection of multicellular hosts through receptor binding and membrane penetration via the canonical endosomal pathway. Unlike totiviruses that infect unicellular hosts, toti-like viruses, including artiviruses, infect invertebrate multicellular hosts and possess an additional surface crown protein (CrP) on their conserved T = 1 icosahedral capsid. The mosquito-specific artivirus Omono River virus (OmRV) also expresses this CrP, which has been suggested to facilitate viral infection and propagation, although it may not be essential. To clarify its role in viral infection, we generated a CrP deletion mutant of OmRV to directly assess its functional significance. The absence of CrP does not affect infectivity in mosquito cells or alter the capsid structure. However, cytopathic effects (CPEs), vesicular virus factories, and viral efficient transmission, mediated by viral release and cell-to-cell spread, are still affected. These findings indicate that CrP is dispensable for OmRV infection but may play a crucial role in viral release, providing important insight into its previously unclear function in the viral life cycle. PubMed: 42066466DOI: 10.1016/j.virol.2026.110922 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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