9T4R
Staphylococcus aureus 70S initiation complex with a natural mRNA
This is a non-PDB format compatible entry.
Summary for 9T4R
| Entry DOI | 10.2210/pdb9t4r/pdb |
| EMDB information | 55526 |
| Descriptor | Large ribosomal subunit protein bL33B, Large ribosomal subunit protein uL2, 16S rRNA, ... (52 entities in total) |
| Functional Keywords | shine-delgarno, anti shine-delgarno, translation initiation, staphylococcus aureus., ribosome |
| Biological source | Staphylococcus aureus More |
| Total number of polymer chains | 52 |
| Total formula weight | 2159253.43 |
| Authors | |
| Primary citation | Kohl, M.P.,Bahena-Ceron, R.,Chane-Woon-Ming, B.,Kompatscher, M.,Erlacher, M.D.,Barchet, C.,von Loeffelholz, O.,Romby, P.,Klaholz, B.P.,Marzi, S. Extended Shine-Dalgarno motifs govern translation initiation in Staphylococcus aureus. Nat Commun, 2026 Cited by PubMed Abstract: Regulation of translation initiation is central to bacterial adaptation, but species-specific mechanisms remain poorly understood. We present high-resolution mapping of translation start sites in Staphylococcus aureus, revealing distinct features of initiation alongside numerous unannotated small ORFs. Our analysis, combined with cryo-EM of a native mRNA-ribosome complex, shows that S. aureus relies on extended, start codon proximal Shine-Dalgarno (SD) interactions, creating specificity against phylogenetically distant bacteria. Several natural S. aureus initiation sites are not correctly decoded by E. coli ribosomes. We identify new and conserved non-canonical start codons, whose regulatory initiation sites contain these characteristic extended SD sequence motifs. Finally, we characterize a novel example of uORF-mediated translational control in S. aureus, demonstrating that translation of a small leader peptide modulates expression of a key biofilm regulator. The described mechanism involves codon rarity, ribosome pausing, and arginine availability, linking nutrient sensing to biofilm formation in this major human pathogen. PubMed: 41680142DOI: 10.1038/s41467-026-69079-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.3 Å) |
Structure validation
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