9T1R

Structure of the PDZ domain of human Inturned

Summary for 9T1R

• Entry DOI: 10.2210/pdb9t1r/pdb
• Descriptor: Protein inturned (2 entities in total)
• Functional Keywords: pdz, gef, inturned, cytosolic protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 28703.38

Authors

Wilmes, S.,Kuemmel, D. (deposition date: 2025-10-21, release date: 2026-05-27)

Primary citation

Wilmes, S.,Brysch, J.,Gelze, C.,Meier, L.,Kummel, D.
The planar cell polarity protein Vangl2 interacts with the PDZ-domains of Scribble but not with a unique PDZ-like domain in Inturned.
Febs Lett., 2026

PubMed Abstract: The proteins Inturned and Fuzzy are members of the tri-longin domain (TLD) RabGEF family and activate the GTPase Rab23 downstream of the core planar cell polarity (PCP) proteins Vangl2 and Prickle. To gain insight into the function of a predicted PDZ domain unique to Inturned among TLD proteins, we performed structural and biochemical characterisations. We show that this domain does not interact with membranes or Vangl2. Instead, we find a phosphorylation-dependent interaction between Vangl2 and a PDZ domain of the apical-basal polarity protein Scribble. A crystal structure of Intu-PDZ reveals a unique PDZ-like fold lacking an interaction site for PDZ-binding motifs. Our data provide new insight into the role of PDZ domains in coordinating cell polarity downstream of Vangl2.

PubMed: 41797376
DOI: 10.1002/1873-3468.70319

Experimental method

X-RAY DIFFRACTION (2.5 Å)