9SN7
Crystal structure of anthocyanin-related glutathione transferase from poplar in complex with quercetin
Summary for 9SN7
| Entry DOI | 10.2210/pdb9sn7/pdb |
| Descriptor | glutathione transferase, Glutathione, 3,5,7,3',4'-PENTAHYDROXYFLAVONE, ... (4 entities in total) |
| Functional Keywords | glutathione, glutathione transferase, anthocyanin, cyanidin, poplar, quercetin, transferase |
| Biological source | Populus trichocarpa (black cottonwood) |
| Total number of polymer chains | 1 |
| Total formula weight | 24949.49 |
| Authors | Didierjean, C.,Favier, F.,Mathiot, S. (deposition date: 2025-09-10, release date: 2026-07-29, Last modification date: 2026-08-05) |
| Primary citation | Morette, L.,Mathiot, S.,Rochoux, S.,Schwander, T.,Schwartz, M.,Nguyen, H.M.,Favier, F.,Buller, R.,Hecker, A.,Didierjean, C. Structural and biochemical insights into an anthocyanin-related glutathione transferase from bilberry and its inhibition by quercetin. Int.J.Biol.Macromol., 376:153396-153396, 2026 Cited by PubMed Abstract: The anthocyanin biosynthetic pathway (ABP), a branch of the phenylpropanoid pathway, is responsible for the production of a wide range of flavonoid compounds in plants. Anthocyanin-related glutathione transferases (arGSTs) have long been proposed to act as non-catalytic carrier proteins, mediating the vacuolar sequestration of anthocyanins. However, recent structural and biochemical evidence has suggested a potential catalytic role for arGSTs in the ABP, notably in catalyzing the dehydration of flavan-3,3,4-triol to anthocyanidin. Despite their importance, only a limited number of arGSTs have been characterized biochemically and structurally. Here, we investigated the arGST isoform from bilberry, one of the richest fruit sources of anthocyanins. We showed that the expression of the arGST gene in bilberry increases during fruit ripening in parallel with other ABP genes and putative transcription factors. Biochemical and structural analyses highlighted the remarkable ability of GSTs to adapt to their substrates and revealed a possible inhibitory effect of quercetin on arGST activity, as quercetin is a by-product of the anthocyanin biosynthetic pathway. PubMed: 42413677DOI: 10.1016/j.ijbiomac.2026.153396 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.4 Å) |
Structure validation
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