9S3N

SARS-CoV-2 nucleocapsid N/C-terminal domain in complex with BCY17628

Summary for 9S3N

• Entry DOI: 10.2210/pdb9s3n/pdb
• Descriptor: Nucleoprotein, BCY17628, 2,4,6-tris(chloromethyl)-1,3,5-triazine, ... (4 entities in total)
• Functional Keywords: hydrolase
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 16417.60

Authors

Brear, P.,Lulla, A.,Dodds, R.,Bezerra, G.,Hyvonen, M. (deposition date: 2025-07-24, release date: 2026-03-04, Last modification date: 2026-03-11)

Primary citation

Shamsabadi, A.,Creamer, A.,Sadler, C.J.,Abdelwahed, A.,Gaynor, K.U.,Demydchuk, Y.,Ivanova-Berndt, G.,Van Rietschoten, K.,Beswick, P.,Chen, L.,Arruda Bezerra, G.,Lulla, A.,Brear, P.,Hyvonen, M.,Skynner, M.J.,Stevens, M.M.
Utilizing Constrained Bicyclic Peptides for In Vitro Diagnostics.
Acs Nano, 20:5928-5939, 2026

PubMed Abstract: Constrained bicyclic peptides () with high affinity for biological targets have emerged as potentially powerful therapeutic agents, particularly for the targeting of cancer receptors. However, their antibody-mimetic properties have yet to be explored for use in diagnostic immunoassays. These synthetically derived compounds serve as biorecognition scaffolds that allow for facile site-selective modification and large-scale production. A phage display screen against various constructs of the SARS-CoV-2 nucleocapsid (N) protein identified several molecules with binding affinities ranging from the micromolar to the low nanomolar range. These molecules were validated in the development of enzyme- and nanozyme-linked immunosorbent assays, as well as enzymatic and colorimetric nanoparticle-based lateral flow immunoassays (LFIA) for the detection of ultralow concentrations of the SARS-CoV-2 N protein. We envision that these moieties enable robust, cost-effective, and large-scale development of ultrasensitive biosensors for a diverse range of biomarkers by leveraging their high binding affinity, minimalistic scaffold, and synthetic accessibility.

PubMed: 41685809
DOI: 10.1021/acsnano.5c19041

Experimental method

X-RAY DIFFRACTION (1.93 Å)