9S2D

KHNYN exPIN nuclease

9S2D の概要

• エントリーDOI: 10.2210/pdb9s2d/pdb
• 分子名称: Protein KHNYN (2 entities in total)
• 機能のキーワード: khnyn, nuclease, antiviral, rna binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42378.45

構造登録者

Youle, R.L.,Taylor, I.A. (登録日: 2025-07-21, 公開日: 2025-12-31)

主引用文献

Youle, R.L.,Lista, M.J.,Brudenell, E.L.,Thompson, B.,Bouton, C.,Morris, E.R.,Neil, S.J.D.,Swanson, C.M.,Taylor, I.A.
KHNYN is a manganese-dependent endoribonuclease required for ZAP-mediated antiviral restriction.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: Zinc finger antiviral protein (ZAP) is a cytoplasmic protein central to host innate immunity to viral infection. ZAP has no intrinsic catalytic activity but inhibits viral replication by binding to CpG dinucleotides in cytoplasmic viral RNA and recruiting other factors to inhibit protein synthesis and target the RNA for degradation. KHNYN is a ZAP-binding protein required for ZAP-restriction of CpG-rich viral genomes. It contains an extended diKH, PIN nuclease, and CUElike domain, each of which are required for ZAP restriction of viral replication. Here, we report a structural, enzymological, and virological study of KHNYN's essential PIN nuclease domain. Our crystal structure reveals an extended PIN domain (ex-PIN) containing a conserved N-terminal arm region required for domain stability and an active site tetra-Asp motif, which are both required for antiviral activity. Unlike the weak activity recently reported for the PIN domain, we demonstrate that the KHNYN ex-PIN domain is a highly active Mn2+-dependent single-stranded RNA endonuclease that cleaves with a preference for ApC, ApA, and UpA dinucleotides. These observations extend our view of KHNYN antiviral activity and suggest an unforeseen role for activation by manganese ions in the ZAP-KHNYN antiviral response.

PubMed: 41404804
DOI: 10.1093/nar/gkaf1360

実験手法

X-RAY DIFFRACTION (2.35 Å)