9RZR

Hen Egg-White Lysozyme (HEWL) complexed with a Lindqvist-type hexavanadate (V6-Hex) polyoxometalate

This is a non-PDB format compatible entry.

Summary for 9RZR

• Entry DOI: 10.2210/pdb9rzr/pdb
• Descriptor: Lysozyme C, Hexavanadate (V6-Hex) polyoxometalate, MALONATE ION, ... (5 entities in total)
• Functional Keywords: complex, polyoxometalate, protein binding, hydrolase
• Biological source: Gallus gallus (chicken)
• Total number of polymer chains: 1
• Total formula weight: 16866.77

Authors

Moussawi, M.A.,Parac-Vogt, T.N. (deposition date: 2025-07-16, release date: 2025-10-01, Last modification date: 2025-12-17)

Primary citation

Kalandia, G.,Declerck, K.,Salazar Marcano, D.E.,Gourmand, R.,Moussawi, M.A.,Parac-Vogt, T.N.
Finding the Key: Binding of Metal-Oxo Clusters to the Enzyme Active Site Enabled by "Click" (Bio)Conjugation.
Angew.Chem.Int.Ed.Engl., 64:e202518349-e202518349, 2025

PubMed Abstract: Molecular metal-oxo nanoclusters with tunable redox and structural properties have emerged as powerful bio-inorganic tools in catalysis, protein crystallization, and therapeutic applications. Despite their potential, interactions between discrete clusters and proteins are predominantly driven by nonspecific intermolecular interactions, which limit precise control over binding sites and functional outcomes. In this work, we introduce a new strategy to achieve site-directed binding of vanadium-based polyoxometalate clusters (POVs) to distinct regions of Hen Egg White Lysozyme (HEWL), an archetypal antimicrobial enzyme. Three novel hybrid POVs were designed and fully characterized, starting from an azide-functionalized cluster (Na-V-N), which was subsequently post-functionalized with a hydrophobic hexyne group (Na-V-Hex) to probe nonpolar interactions, and α-d-mannopyranoside (Na-V-Man) to mimic the protein's natural substrate. Structural and spectroscopic analyses demonstrated that, in contrast to conventional nonhybrid POV clusters which bind nonspecifically to peripheral positively charged protein patches, the hybrid POVs achieve distinct binding behaviors. Specifically, Na-V-N and Na-V-Man selectively target the glycosidic pocket, resembling the binding of the protein's natural substrate, while Na-V-Hex exhibits an unprecedented crystallization of two POV clusters in close proximity, which wrap around the protein surface. These findings highlight that strategic organic functionalization can circumvent electrostatic barriers to achieve site-selective cluster-protein interactions, thus opening new avenues for the application of metal-oxo clusters in biotechnology, drug delivery, and medicine.

PubMed: 40985763
DOI: 10.1002/anie.202518349

Experimental method

X-RAY DIFFRACTION (1.27 Å)