9RUL
CdmB methyltransferase involved in the anaerobic dehalogenation of haloalkanes soaked with dichloromethane
This is a non-PDB format compatible entry.
Summary for 9RUL
| Entry DOI | 10.2210/pdb9rul/pdb |
| Descriptor | CdmB from Acetobacterium malicum subsp. dehalogenans, SODIUM ION, CHLORIDE ION, ... (12 entities in total) |
| Functional Keywords | chloromethane conversion, acetogenesis, anaerobic bacteria, dehalogenation, methyl-transfer, dichloromethane, transferase |
| Biological source | Acetobacterium malicum subsp. dehalogenans DSM 11527 |
| Total number of polymer chains | 2 |
| Total formula weight | 101884.91 |
| Authors | Wagner, T.,Lemaire, O.N. (deposition date: 2025-07-04, release date: 2026-06-03, Last modification date: 2026-06-10) |
| Primary citation | Bernhardt, J.,Hofmann, L.K.R.,Klemm, P.,Paczia, N.,Lemaire, O.N.,Vuilleumier, S.,Wagner, T.,Kurth, J.M. Identification and characterisation of an elusive bacterial enzyme system for chloromethane dehalogenation. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: Chloromethane, a toxic gas primarily produced naturally, contributes to stratospheric ozone destruction. The anaerobic acetogen Acetobacterium dehalogenans can utilise chloromethane as a carbon and energy source, but the associated dehalogenase/methyltransferase has remained elusive. Through comparative transcriptomics we identify a gene cluster, cdmBCA, which encodes a corrinoid-dependent methyltransferase system distinct from the characterised Cmu system used for chloromethane degradation in aerobic methylotrophs. Biochemical characterisation reveals that the Cdm system reacts with other haloalkanes, but not with methoxylated aromatics, unlike closely related O-demethylases. X-ray structural analysis of the protein CdmB shows a hydrophobic channelling system directing haloalkanes towards cobalamin-dependent activation. Homologous proteins are found in anaerobic prokaryotes, particularly within the phyla Bacillota and Asgardarchaeota, suggesting previously unidentified microbial transformation of chloromethane in the environment. Discovery of the Cdm dehalogenation/methyltransferase system sheds light on the microbial contribution to the global chloromethane cycle. PubMed: 42218128DOI: 10.1038/s41467-026-73764-z PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.62 Å) |
Structure validation
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