9RP3

Structure of Avast type 5 activator (Gp316, JADA jumbophage protein)

Summary for 9RP3

• Entry DOI: 10.2210/pdb9rp3/pdb
• EMDB information: 54139
• Descriptor: Gp316/JADA (1 entity in total)
• Functional Keywords: jumbophage, gp316, jada, avast5, dimer, unknown function
• Biological source: Pseudomonas phage vB_PA32_GUMS
• Total number of polymer chains: 2
• Total formula weight: 160422.69

Authors

Pacesa, M.,Muralidharan, A.,Brouns, S.J.J. (deposition date: 2025-06-23, release date: 2025-07-02, Last modification date: 2026-02-18)

Primary citation

Muralidharan, A.,Costa, A.R.,Fierlier, D.,van den Berg, D.F.,van den Bossche, H.,Zoumaro-Djayoon, A.D.,Rodriguez-Molina, A.,Pabst, M.,Pacesa, M.,Correia, B.E.,Brouns, S.J.J.
Molecular basis for anti-jumbo phage immunity by AVAST type 5.
Mol.Cell, 2026

PubMed Abstract: Jumbo phages protect their genomes from DNA-sensing bacterial defense systems by enclosing them within vesicles and nucleus-like compartments. Very little is known about defense systems specialized to counter these phages. Here, we show that AVAST type 5 (Avs5) systems, part of the signal transduction ATPases of numerous domains (STAND) superfamily, confer conserved immunity against jumbo phages. Using fluorescence microscopy and biotin proximity labeling, we demonstrate that Avs5 localizes to early infection vesicles, where it senses an essential, early-expressed phage protein named JADA (Jumbo phage Avs5 Defense Activator). Recognition of phage infection triggers the Sir2-like effector domain of Avs5 across three Avs5 clades, resulting in rapid NAD hydrolysis, disruption of phage nucleus formation, and arrest of infection. These findings reveal a spatially coordinated bacterial immune strategy that targets an early vulnerability in jumbo phage infection.

PubMed: 41653918
DOI: 10.1016/j.molcel.2026.01.004

Experimental method

ELECTRON MICROSCOPY (2.1 Å)