9RM9

Cryo-EM structure of alphaM/beta2 headpiece complex without alphaM I-domain - the consensus map from alphaM/beta2:C3d-anti-CR3-Nb headpiece complex

Summary for 9RM9

• Entry DOI: 10.2210/pdb9rm9/pdb
• EMDB information: 52188 52189 52190 52191 54052
• Descriptor: Isoform 2 of Integrin alpha-M, Integrin beta-2, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: phagocytosis, integrin, opsonisation, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 140446.77

Authors

Fruergaard, M.U.,Andersen, G.R. (deposition date: 2025-06-18, release date: 2026-04-29, Last modification date: 2026-07-01)

Primary citation

Lorentzen, J.,Fruergaard, M.U.,Lukacsi, S.,Jorgensen, M.H.,van Veghel, T.L.G.,Jensen, R.K.,Pietrzak-Lichwa, K.J.,Bajtay, Z.,Horejsi, V.,Flygaard, R.K.,Vorselen, D.,Mortensen, S.A.,Andersen, G.R.
Three cryo-EM structures of complement C3d-bound alpha M beta 2 reveal an unexpected layer of dynamics for alpha I-containing integrin receptors.
Sci Adv, 12:eaea7241-eaea7241, 2026

PubMed Abstract: Integrins are heterodimeric membrane proteins acting as mechanosensing receptors. Nine human α-subunits contain a ligand binding αI domain, but how ligands activate αI integrins are not understood. We present cryo-EM structures of the αI integrin αβ in complex with the C3d ligand. The ligand-bound αI domain appears to have two major opposite orientations relative to the β subunit. Ligand binding induces an ordered conformation of the α internal ligand region that is tightly packed between the α β-propeller and the β βI-domain. Recognition of the internal ligand induces an open βI conformation practically identical to that of ligand-bound αI-less integrins confirming that ligand binding and signaling are coupled by a universal mechanism across all integrins. Integration of our findings with prior data allows us to propose a model for C3dg/iC3b-bound αβ in the phagocytotic cup and outline mechanistic models for external ligand-induced activation of αβ.

PubMed: 42102216
DOI: 10.1126/sciadv.aea7241

Experimental method

ELECTRON MICROSCOPY (2.6 Å)