9RIB
Crystal structure of C278S mutant of mouse CDC14A
Summary for 9RIB
| Entry DOI | 10.2210/pdb9rib/pdb |
| Descriptor | Dual specificity protein phosphatase CDC14A, GLYCEROL, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | cdc14a, dual specificity phosphatase, hearing loss, structural-functional relationship, cell cycle |
| Biological source | Mus musculus (house mouse) |
| Total number of polymer chains | 2 |
| Total formula weight | 80439.65 |
| Authors | Shabbir, K.,Jackisch, G.,Knecht, W.,Wilson, E.,Dong, L.,Friedman, T.B.,Imtiaz, A.,Logan, D.T. (deposition date: 2025-06-11, release date: 2025-12-10, Last modification date: 2026-01-07) |
| Primary citation | Shabbir, K.,Jackisch, G.,Belyantseva, I.A.,Imran, M.,Naz, S.,Sele, C.,Murina, V.,Knecht, W.,Friedman, T.B.,Logan, D.T.,Imtiaz, A. A truncated CDC14A retains catalytic structure and phosphatase activity preserving male fertility but causes nonsyndromic deafness. J.Biol.Chem., 302:110982-110982, 2025 Cited by PubMed Abstract: Pathogenic variants of human CDC14A (cell division cycle 14A) are associated either with nonsyndromic deafness DFNB32 or hearing impairment infertile male syndrome (HIIMS). The 623-residue CDC14A protein has two globular domains (residues 17-152 and 217-325) and a 278-residue C-terminal intrinsically disordered region (IDR). To date, 16 recessive variants of human CDC14A are associated with hearing loss. Variants affecting the globular N-terminal domains of human CDC14A are associated with HIIMS, whereas mutations in the IDR cause nonsyndromic deafness DFNB32. Here, we tested the hypothesis that the human CDC14A c.1033C>T variant, segregating with nonsyndromic deafness in family PKSN10, introduces a premature translation stop codon (p.R345∗), yet the mRNA escapes nonsense-mediated decay and produces sufficient active phosphatase to allow for male fertility. Quantitative analyses of CDC14A mRNA in blood leukocytes from the PKSN10 family showed that CDC14A transcripts are stable, including p.R345∗ transcripts, which evade nonsense-mediated decay. To further test this hypothesis, we performed biochemical and structural characterizations of truncated CDC14A (ΔC-CDC14A) protein retaining only residues 1 to 345. Kinetic functional studies and X-ray crystallographic findings of purified ΔC-CDC14A protein indicate that it retains structural integrity and phosphatase activity. Molecular genetic reports of DFNB32 and HIIMS, taken together with structural and functional data in this study, indicate that phosphatase activity of ΔC-CDC14A containing the two globular domains is sufficient for male fertility but insufficient for normal hearing. In addition, we show that the C-terminal IDR of CDC14A is required for normal hearing, likely because it is necessary for normal localization of CDC14A in hair cells. PubMed: 41308992DOI: 10.1016/j.jbc.2025.110982 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.62 Å) |
Structure validation
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