9RAX
The L1 amyloid-beta(1-40)fibril in the presence of anle138b (pre-treatment)
Summary for 9RAX
| Entry DOI | 10.2210/pdb9rax/pdb |
| EMDB information | 53882 |
| Descriptor | Amyloid-beta A4 protein (1 entity in total) |
| Functional Keywords | amyloid-beta, fibril, anle138b, protein fibril |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 10 |
| Total formula weight | 43358.52 |
| Authors | Frieg, B.,Han, M.,Griesinger, C.,Schroeder, G.F. (deposition date: 2025-05-21, release date: 2025-10-15) |
| Primary citation | Han, M.,Frieg, B.,Matthes, D.,Leonov, A.,Ryazanov, S.,Giller, K.,Nimerovsky, E.,Stampolaki, M.,Xue, K.,Overkamp, K.,Dienemann, C.,Riedel, D.,Giese, A.,Becker, S.,de Groot, B.L.,Schroder, G.F.,Andreas, L.B.,Griesinger, C. Anle138b binds predominantly to the central cavity in lipidic A beta 40 fibrils and modulates fibril formation. Nat Commun, 16:8850-8850, 2025 Cited by PubMed Abstract: Alzheimer's disease is a specific neurodegenerative disorder, distinct from normal aging, with a growing unmet medical need. It is characterized by the accumulation of amyloid plaques in the brain, primarily consisting of amyloid beta (Aβ) fibrils. Therapeutic antibodies can slow down the disease, but are associated with potential severe side effects, motivating the development of small molecules to halt disease progression. This study investigates the interaction between the clinical drug candidate small molecule anle138b and lipidic Aβ₄₀ fibrils of type 1 (L1). L1 fibrils were previously shown to closely resemble fibrils from Alzheimer's patients. Using high-resolution structural biology techniques, including cryo-electron microscopy (cryo-EM), nuclear magnetic resonance (NMR) spectroscopy enhanced by dynamic nuclear polarization (DNP), and molecular dynamics (MD) simulations, we find that anle138b selectively binds to a cavity within the fibril. This structural insight provides a deeper understanding of a potential drug-binding mechanism at the atomic level and may inform the development of therapies and diagnostic approaches. In addition, anle138b reduces fibril formation in the presence of lipids by approximately 75%. This may suggest a mechanistic connection to its previously reported activity in animal models of Alzheimer's disease. PubMed: 41044155DOI: 10.1038/s41467-025-64443-6 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.76 Å) |
Structure validation
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