Summary for 9R86
| Entry DOI | 10.2210/pdb9r86/pdb |
| EMDB information | 53805 |
| Descriptor | Major vault protein (1 entity in total) |
| Functional Keywords | complex, vault, large protein, structural protein |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 39 |
| Total formula weight | 3878657.87 |
| Authors | Lovestam, S.L.,Scheres, S.H.W. (deposition date: 2025-05-15, release date: 2025-07-02, Last modification date: 2025-10-15) |
| Primary citation | Lovestam, S.,Scheres, S.H.W. Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps. Structure, 33:1643-, 2025 Cited by PubMed Abstract: The vault protein is expressed in most eukaryotic cells, where it is assembled on polyribosomes into large hollow barrel-shaped complexes. Despite its widespread and abundant presence in cells, the biological function of the vault remains unclear. In this study, we describe the cryo-EM structure of vault particles that were imaged as a contamination of a preparation to extract tau filaments from brain tissue of an individual with progressive supranuclear palsy (PSP). We identify a mechanism of symmetry mismatch at the caps of the vault, from 39-fold to 13-fold symmetry, where two out of three monomers are sequentially excluded from the cap, resulting in a narrow, greasy pore at the tip of the vault. Our structure offers valuable insights for engineering carboxy-terminal modifications of the major vault protein (MVP) for potential therapeutic applications. PubMed: 40803316DOI: 10.1016/j.str.2025.07.014 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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