9R4Y
15 protofilament P. falciparum GMPCPP microtubule
Summary for 9R4Y
| Entry DOI | 10.2210/pdb9r4y/pdb |
| EMDB information | 53571 53572 |
| Descriptor | Tubulin alpha chain, Tubulin beta chain, GUANOSINE-5'-TRIPHOSPHATE, ... (5 entities in total) |
| Functional Keywords | malaria, parasite, cytoskeleton, microtubule, gmpcpp, structural protein |
| Biological source | Plasmodium falciparum 3D7 More |
| Total number of polymer chains | 2 |
| Total formula weight | 101239.60 |
| Authors | Bangera, M.,Moores, C.A. (deposition date: 2025-05-08, release date: 2026-03-11, Last modification date: 2026-03-18) |
| Primary citation | Bangera, M.,Wu, J.,Beckett, D.,Fachet, D.,Ferreira, J.L.,Voth, G.A.,Reber, S.,Moores, C.A. Adaptations in Plasmodium tubulin determine distinct microtubule architectures, mechanics and drug susceptibility. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: Microtubules are ubiquitous yet diverse cytoskeleton filaments. However, tubulin conservation presents challenges in understanding the origins of diverse microtubule architectures. The mechanisms by which microtubule architecture varies through the life cycle of the malaria-causing parasite Plasmodium are not understood and provide a valuable framework for exploring how intrinsic properties of tubulin contribute to architectural variety. Using parasite-purified tubulin, we determine P. falciparum microtubule structures by cryo-electron microscopy. Parasite-specific sequences change the tubulin dimer structure, suggesting how drug susceptibility and polymer properties are modified. Within the P. falciparum microtubule, lateral contacts are smaller but stronger, and the lattice is stiffer than in brain microtubules. Non-canonical microtubule architectures found in parasites are highly similar to those observed in vitro, validating the physiological relevance of these properties. Our findings show how evolutionary adaptation of tubulin modulates the material properties of the microtubule cytoskeleton. PubMed: 41786731DOI: 10.1038/s41467-026-70181-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.9 Å) |
Structure validation
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