9QZC
Proximal A-C linker of Tetrahymena centriole, one repeating unit
This is a non-PDB format compatible entry.
Summary for 9QZC
| Entry DOI | 10.2210/pdb9qzc/pdb |
| EMDB information | 53467 |
| Descriptor | WD repeat WRAP73-like protein, putative, Unknown Protein Chain, Centrosomal protein, putative, ... (15 entities in total) |
| Functional Keywords | centriole, basal body, centrosome, structural protein |
| Biological source | Tetrahymena thermophila More |
| Total number of polymer chains | 18 |
| Total formula weight | 757563.27 |
| Authors | Cai, B.,Xu, J.W.,Luo, L.,Aarts, E.,Leitner, A.,Ishikawa, T.,Beltro, P.,Pilhofer, M.,Wieczorek, M. (deposition date: 2025-04-22, release date: 2025-09-17, Last modification date: 2025-10-22) |
| Primary citation | Cai, B.,Xu, J.,Collet, E.H.,Aarts, E.,Luo, L.,Leitner, A.,Ishikawa, T.,Beltrao, P.,Pearson, C.G.,Pilhofer, M.,Wieczorek, M. Structure and assembly of the A-C linker connecting microtubule triplets in centrioles. Sci Adv, 11:eady3689-eady3689, 2025 Cited by PubMed Abstract: Centriole assembly involves the coordination of centriolar modules. One module is the A-C linker, an enigmatic protein assembly connecting the A-microtubule of one microtubule triplet to the C-microtubule of the neighboring triplet. Here, we integrated biochemistry, multiscale cryo-electron microscopy, and AlphaFold modeling to investigate the architecture of the centriole. Using an improved centriole isolation method, we determined the structure of the A-C linker bound to microtubule triplets, which revealed how the A-C linker cross-links microtubules and integrates with the B-C junction. We found marked changes in the structure and composition of the A-C linker that correlate with the presence of other centriolar modules, including the pinhead, cartwheel, and inner scaffold. Our findings show that the A-C linker is a highly integrated component of the centriole whose polymorphism may orchestrate the assembly of spatially distinct centriolar modules, and provide a framework for dissecting the biology of centrioles. PubMed: 41061066DOI: 10.1126/sciadv.ady3689 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.8 Å) |
Structure validation
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