9QTG
Simkania negevensis CE-clan virulence factor SnCE1 in complex with hsSUMO1
Summary for 9QTG
| Entry DOI | 10.2210/pdb9qtg/pdb |
| Descriptor | Deubiquitinase and deneddylase ChlaDub2, Small ubiquitin-related modifier 1, ACETATE ION, ... (6 entities in total) |
| Functional Keywords | cysteine protease, ce-clan, ubl protease, desumoylase, sumo pathway, virulence factor, bacterial virulence, pathogenesis, hydrolase |
| Biological source | Simkania negevensis Z More |
| Total number of polymer chains | 2 |
| Total formula weight | 37666.92 |
| Authors | Schmoeker, O.,Girbardt, B.,Palm, G.J.,Hoppen, J.,Schulze, S.,Lammers, M. (deposition date: 2025-04-08, release date: 2026-04-08, Last modification date: 2026-05-06) |
| Primary citation | Schmoker, O.,Girbardt, B.,Schulze, S.,Palm, G.J.,Berndt, L.,Hoppen, J.,Kara, N.,Schops, X.,Al-Abdulla, R.,Garz, K.,Junker, H.,Wolfgramm, S.,Steil, L.,Hentschker, C.,Schoknecht, K.,Mayer, L.M.,Speth, L.,Lachmayer, V.,Dorr, M.,Kemnitz, S.,Muller, S.,Lackmann, J.W.,Kruger, M.,Hofmann, K.,Bornscheuer, U.T.,Volker, U.,Kruger, E.,Kozjak-Pavlovic, V.,Lammers, M. Reprogramming of bacterial virulence by lysine acetylation. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: Gram-negative bacteria use a plethora of virulence factors to infect eukaryotic cells. CE-clan protease-related virulence factors were reported to act as deubiquitinases/ubiquitin-like specific proteases. Some have an additional acetyl-transferase activity. The molecular mechanisms underlying this dual activity and the physiological consequences are only marginally understood. Here, we report crystal structures for the Simkania negevensis virulence factor SnCE1 in apo-states and in complex with SUMO1. We confirm SnCE1 acting as an efficient deSUMOylase and discover an intrinsic autoacetyltransferase activity. Acetylation impairs SnCE1 tetramer formation structurally being incompatible with SUMO1 binding. We provide a model for regulation of SnCE1-mediated virulence by lysine acetylation modulating autoproteolytic processing and its subcellular distribution in the host cell. SnCE1 localizes to the endoplasmic reticulum in human cells and increases fragmentation of mitochondria. Our data provide mechanistic insights into how lysine acetylation of virulence factors is used to reprogram virulence adjusting it to the host cells' metabolic state. PubMed: 42045228DOI: 10.1038/s41467-026-72244-8 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.55 Å) |
Structure validation
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