9QHX
ATP-bound E178Q Wzm-Wzt from Mycobacterium abscessus in nanodiscs
Summary for 9QHX
| Entry DOI | 10.2210/pdb9qhx/pdb |
| Related | 9QFX 9QGU 9QH1 9QHJ 9QHK 9QHV 9QHW |
| EMDB information | 53123 53148 53152 53171 53172 53180 53181 53182 |
| Descriptor | ABC transporter permease, ABC transporter ATP-binding protein, MAGNESIUM ION, ... (4 entities in total) |
| Functional Keywords | abc transporter type v abc transporter arabinogalactan precursor lipid-linked galactan exporter, membrane protein |
| Biological source | Mycobacteroides abscessus More |
| Total number of polymer chains | 4 |
| Total formula weight | 128466.87 |
| Authors | Garaeva, A.A.,Fabianova, V.,Savkova, K.,Huszar, S.,Xue, X.,Lowary, T.L.,Mikusova, K.,Seeger, M.A. (deposition date: 2025-03-16, release date: 2026-03-11, Last modification date: 2026-04-08) |
| Primary citation | Garaeva, A.A.,Fabianova, V.,Savkova, K.,Huszar, S.,Xue, X.,Lowary, T.L.,Mikusova, K.,Seeger, M.A. Structural basis of lipid-linked galactan export by the mycobacterial ABC transporter Wzm-Wzt. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: Mycobacteria, including Mycobacterium tuberculosis, possess a unique cell envelope containing arabinogalactan, a heteropolysaccharide critical for cell wall integrity and target of several tuberculosis drugs. The cytosolic precursor of arabinogalactan, lipid-linked galactan (LLG), is translocated across the plasma membrane by the essential ABC transporter Wzm-Wzt through a molecular mechanism that is poorly understood. Here, we present a series of cryo-EM structures of Wzm-Wzt from Mycobacterium abscessus, representing different conformations of the transport cycle. Conserved residues lining the proposed LLG translocation pathway were investigated by three orthologous functional assays, revealing that the cytosolic gate helix (GH) plays a key functional role in polysaccharide transport. Our data suggests that the hydrophobic polyprenyl-moiety is translocated first, followed by the galactan-polysaccharide, which requires Wzm-Wzt to open a continuous channel through which the sugar chain is ratcheted at the expense of ATP hydrolysis. Our results provide a rational basis for the development of drugs that inhibit mycobacterial cell wall biosynthesis. PubMed: 41839883DOI: 10.1038/s41467-026-70429-9 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.47 Å) |
Structure validation
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