9QEV
A native carbonic anhydrase from Caloramator australiacus
Summary for 9QEV
| Entry DOI | 10.2210/pdb9qev/pdb |
| Descriptor | Carbonic anhydrase, family 3, ZINC ION, SODIUM ION, ... (5 entities in total) |
| Functional Keywords | thermophilic bacteria, thermostability, metalloenzyme, metal binding protein |
| Biological source | Caloramator australicus |
| Total number of polymer chains | 3 |
| Total formula weight | 58283.68 |
| Authors | |
| Primary citation | Bodourian, C.S.,Imran, M.,Georgakis, N.D.,Papageorgiou, A.C.,Labrou, N.E. Structural and functional characterization of a metagenomically derived gamma-type carbonic anhydrase and its engineering into a hyperthermostable esterase. Protein Sci., 34:e70396-e70396, 2025 Cited by PubMed Abstract: The 16S microbial community profiling of a metagenomics library from geothermal spring at Lisvori (Lesvos island, Greece) enabled the identification of a putative sequence exhibiting 95% identity to the γ-type carbonic anhydrase (γ-CA) from Caloramator australicus (γ-CaCA). The sequence of γ-CaCA was amplified by PCR, cloned, and expressed in E. coli. Activity assays showed that γ-CaCA possesses very low, but detectable, anhydrase activity, while exhibiting no measurable esterase activity. Differential scanning fluorimetry (DSF) revealed that the enzyme shows high thermal stability with a melting temperature (T) approximately 65-75°C in the pH range between 5.5 and 9.0. The structure of γ-CaCA was determined by X-ray crystallography at 1.11 Å resolution, the highest resolution reported so far for a γ-CA. The enzyme was crystallized as a trimer in the crystallographic asymmetric unit and contains three zinc-binding sites, one at each interface of neighboring subunits of the trimer. Structure-based rational design enabled the design and creation of a mutant enzyme (γ-CaCAmut) which possessed a heptapeptide insertion at the active-site loop and two-point mutations. Kinetic analysis demonstrated that γ-CaCAmut was successfully converted into a catalytically active esterase indicating successful activity gain through structure-guided engineering. The thermostability of γ-CaCAmut was significantly increased, aligning with the thermostability typically observed in hyperthermostable enzymes. X-ray crystallographic analysis of the γ-CaCAmut structure at 2.1 Å resolution, provided detailed structural insights into how the mutations impact the overall enzyme structure, function, and thermostability. These findings provide valuable structural and functional insights into γ-CAs and demonstrate a strategy for converting an inactive enzyme into a catalytically active form through rational design. PubMed: 41294346DOI: 10.1002/pro.70396 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.11 Å) |
Structure validation
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