Summary for 9QE0
| Entry DOI | 10.2210/pdb9qe0/pdb |
| Related | 9qe1 |
| EMDB information | 53035 53036 |
| Descriptor | JetC, JetB, JetA (3 entities in total) |
| Functional Keywords | smc complexes, wadjet, jetabcd, dna loop extrusion, dna binding protein |
| Biological source | Neobacillus vireti LMG 21834 More |
| Total number of polymer chains | 8 |
| Total formula weight | 859214.60 |
| Authors | Roisne-Hamelin, F.,Gruber, S. (deposition date: 2025-03-07, release date: 2025-06-25, Last modification date: 2025-09-17) |
| Primary citation | Roisne-Hamelin, F.,Liu, H.W.,Gruber, S. Structure of a type II SMC Wadjet complex from Neobacillus vireti. Structure, 33:1498-, 2025 Cited by PubMed Abstract: Structural maintenance of chromosome complexes are essential DNA-folding motors that facilitate critical cellular functions, including chromosome segregation and DNA repair. Wadjet systems are prokaryotic SMC complexes specialized in cellular immunity against plasmids. Type I Wadjet systems restrict plasmids via a DNA extrusion-cleavage reaction. Two other Wadjet types (II and III) have also been identified, however, their molecular characteristics are unclear. Here, we reconstituted a representative type II Wadjet system from Neobacillus vireti. We show that this system shares substrate selection and cleavage properties with type I but exhibits distinctive structural features, including a long elbow-distal coiled coil, a channel-less hinge, and a tandem KITE subunit. These features help identify the common and distinguishing architectural elements in the family of Wadjet systems and raise intriguing questions about the evolution of prokaryotic SMC complexes. PubMed: 40628265DOI: 10.1016/j.str.2025.06.004 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (6.71 Å) |
Structure validation
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