9PRP

N-terminal domain of E. coli MutL bound to NP660

This is a non-PDB format compatible entry.

Summary for 9PRP

• Entry DOI: 10.2210/pdb9prp/pdb
• Descriptor: DNA mismatch repair protein MutL, 5-(cyclopentyloxy)benzene-1,3-diol (3 entities in total)
• Functional Keywords: dna repair, ghkl atpase, mismatch repair protein, mutl, dna binding protein
• Biological source: Escherichia coli
• Total number of polymer chains: 2
• Total formula weight: 78987.66

Authors

Rodriguez Gonzalez, J.,Guarne, A.,Blagg, J. (deposition date: 2025-07-24, release date: 2026-05-06)

Primary citation

Blagg, J.,Riou, P.,Hervieu, A.,Piumatti, E.,Rodriguez-Plata, M.T.,Battuello, P.,Peall, A.,Amodio, V.,Vitiello, P.P.,Nightingale, D.J.H.,Bago, R.,Tongue, P.,Slater, T.,Parmar, K.,Patel, P.,Rodriguez Gonzalez, J.,Clark, D.E.,Langley, G.W.,Nichols, C.,Guarne, A.,Winship, P.C.M.,Baker, M.,Drysdale, M.,Germano, G.,Bardelli, A.
Pharmacological inhibition of PMS2 induces MMR deficiency and response to immune checkpoint blockade.
Cancer Discov, 2026

PubMed Abstract: DNA mismatch repair (MMR) detects and corrects post-replicative DNA alterations; it is deregulated in up to 20% of human cancers. MMR-deficient (MMR-d) cancers display increased tumour mutational burden (TMB), microsatellite instability (MSI) and are eligible for checkpoint inhibitor (CPI) immunotherapy which commonly elicits durable responses. We reasoned that pharmacological blockade of MMR could broaden the patient population eligible for immunotherapy. Here we reveal MMR protein PMS2 as a druggable target and describe the discovery and characterisation of first-in-class small molecule MMR pathway modulator NP1867. In vitro treatment of murine cancer cells abrogates MMR function and elicits an MMR-d genotype including increased TMB, MMR-d mutational signatures, and MSI-High (MSI- H) status. Inoculation of syngeneic immunocompetent mice with cancer cells pretreated with NP1867 leads to CPI sensitivity, tumour growth delay, and complete responses. For the first time, we demonstrate pharmacological targeting of MMR to proactively rewire the tumour-host relationship for therapeutic purposes.

PubMed: 42013405
DOI: 10.1158/2159-8290.CD-26-0003

Experimental method

X-RAY DIFFRACTION (2.1 Å)