9PIB

Crystal structure of the A/Puerto Rico/8/1934 (H1N1) influenza virus hemagglutinin in complex with fusion inhibitor CLIPS peptide CP121132 (CP-S1)

Summary for 9PIB

• Entry DOI: 10.2210/pdb9pib/pdb
• Descriptor: Hemagglutinin, 1,3,5-trimethylbenzene, Hemagglutinin HA2 chain, ... (11 entities in total)
• Functional Keywords: influenza virus, hemagglutinin, glycoprotein, n-glycosylation, viral protein
• Biological source: Influenza A virus (A/Puerto Rico/8/1934(H1N1)); More
• Total number of polymer chains: 12
• Total formula weight: 238760.74

Authors

Kadam, R.U.,Wilson, I.A. (deposition date: 2025-07-10, release date: 2026-03-18, Last modification date: 2026-04-08)

Primary citation

Kadam, R.U.,Juraszek, J.,Brandenburg, B.,Garg, D.,Zhu, X.,Jongeneelen, M.,Tang, C.,Schepens, W.B.G.,Buyck, C.,Stoops, B.,Vermond, J.,Vogels, R.,Friesen, R.H.E.,van Dongen, M.J.P.,Wilson, I.A.
Small molecule-constrained paratope mimetic bicyclic peptides as potent inhibitors of group 1 and 2 influenza A virus hemagglutinins.
Proc.Natl.Acad.Sci.USA, 123:e2537533123-e2537533123, 2026

PubMed Abstract: Influenza continues to be a major threat to global health and a substantial economic burden. Innovative strategies are needed to tackle the growing resistance to established influenza therapeutics and to develop new therapeutics with novel mechanisms of action. Previous peptide and small molecule designs have been successful only against influenza group 1 hemagglutinin (HA). Here, we report on a CLIPS (Chemical Linkage of Peptides onto Scaffolds)-based approach to design potent peptidic inhibitors of influenza A viruses that now extend to both group 1 and group 2 HAs. This approach merges features of antibodies and small molecules to design constrained bicyclic peptides that engage the highly conserved HA stem. The heavy-chain complementarity-determining region 3 (HCDR3) of human broadly neutralizing antibody FI6v3 was grafted onto functionalized small molecule scaffolds. The designed peptides exhibited in vitro heterosubtypic cross-reactivity in binding to group 1 (H1 and H5) and group 2 (H3 and H7) HAs and in neutralization of H1N1, H5N1, and H7N3 viruses. A crystal structure of the bicyclic peptide with HA from H1N1 A/Puerto Rico/8/1934 (H1/PR8) at 2.35 Å resolution revealed that the designed peptide faithfully mimics the binding mode and functionality of the parent antibody FI6v3 to the highly conserved stem epitope. These structural and functional data illustrate how both group 1 and group 2 influenza A viruses can now be targeted by constrained peptidic ligands that should aid in development of pan-influenza therapeutics.

PubMed: 41875158
DOI: 10.1073/pnas.2537533123

Experimental method

X-RAY DIFFRACTION (2.35 Å)