9PH0
Crystal structure of MAR1 from Gelatoporia subvermispora
Summary for 9PH0
| Entry DOI | 10.2210/pdb9ph0/pdb |
| Descriptor | MAR1, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | mar1, white-rot fungi, lignin, oxidoreductase |
| Biological source | Gelatoporia subvermispora B |
| Total number of polymer chains | 4 |
| Total formula weight | 178779.18 |
| Authors | Mathews, I.I.,Kuatsjah, E.,Schwartz, A.,Sarangi, R.,McGeehan, J.E.,Salvachua, D. (deposition date: 2025-07-08, release date: 2026-06-17) |
| Primary citation | Schwartz, A.,Kuatsjah, E.,Mathews, I.I.,Mitchell, H.D.,Purvine, S.O.,Eder, E.K.,Hoyt, D.W.,Markillie, L.M.,Burnet, M.C.,Woodworth, S.P.,Ingraham, M.A.,Ramirez, K.J.,Monteiro, L.M.O.,Sarangi, R.,McGeehan, J.E.,Salvachua, D. Catabolism of lignin-related methoxylated compounds in white-rot fungi utilizes non-canonical oxidoreductases. Cell Rep, 45:117428-117428, 2026 Cited by PubMed Abstract: White-rot fungi (WRF) are the most effective lignin-degrading organisms in nature. Lignin is a highly methoxylated plant biopolymer, yet the pathways WRF use to metabolize methoxylated aromatic monomeric compounds as carbon sources remain unidentified. Using systems biology, we elucidate the intracellular catabolism of vanillate-a monomethoxylated aromatic compound-by Gelatoporia subvermispora and Trametes versicolor. We identify and biochemically validate a four-enzyme pathway that converts vanillate into β-ketoadipate, which enters central carbon metabolism. Unlike bacteria, which demethylate vanillate before ring-cleavage by intradiol dioxygenases, WRF employ oxidative decarboxylation followed by extradiol dioxygenase-mediated cleavage. A previously uncharacterized hydrolase is also shown to catalyze the terminal step of this pathway. Biochemical and structural approaches reveal non-canonical enzymes, including a highly substrate-specific extradiol dioxygenase and a metal-free, promiscuous reductase that acts on both methoxylated and non-methoxylated intermediates. These findings highlight distinct fungal strategies for aromatic degradation, offering insights into lignin valorization and wood decay mechanisms. PubMed: 42217187DOI: 10.1016/j.celrep.2026.117428 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.79 Å) |
Structure validation
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