9P4C

Crystal structure of Mesothelin C-terminal peptide-RO4 Fab complex

Summary for 9P4C

Entry DOI	10.2210/pdb9p4c/pdb
Descriptor	Light chain of the RO4 Fab fragment, Heavy chain of the RO4 fab fragment, Mesothelin, cleaved form, ... (5 entities in total)
Functional Keywords	mesothelin, antibody, immune system, antitumor protein
Biological source	Oryctolagus cuniculus More
Total number of polymer chains	3
Total formula weight	46353.88
Authors	Zhan, J.,Maslanka, C.,Xia, D. (deposition date: 2025-06-16, release date: 2025-11-12, Last modification date: 2026-02-04)
Primary citation	Onda, M.,Liu, X.,Liu, W.,Zhan, J.,Maslanka, C.A.,Xia, D.,Ho, M.,Pastan, I. RO4, a high-affinity humanized antibody against the juxtamembrane region of mesothelin for targeted cancer therapy. Antib Ther, 9:1-12, 2026 Cited by PubMed Abstract: Mesothelin (MSLN) is a surface antigen highly expressed in several solid tumors, including mesothelioma, ovarian, and pancreatic cancers. However, therapeutic efficacy of MSLN-targeted agents is often compromised by shed MSLN (SM), which acts as a soluble decoy and accumulates in tumor microenvironments, reducing antibody engagement at the tumor surface. PubMed: 41550974 DOI: 10.1093/abt/tbaf022 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.52 Å)

Structure validation