9P3I
High-resolution in situ ANDV single tetramer structure
This is a non-PDB format compatible entry.
Summary for 9P3I
| Entry DOI | 10.2210/pdb9p3i/pdb |
| EMDB information | 71241 |
| Descriptor | Glycoprotein N, Glycoprotein C, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total) |
| Functional Keywords | hantavirus, andv, viral glycoprotein, tetramer, viral protein |
| Biological source | Orthohantavirus andesense More |
| Total number of polymer chains | 8 |
| Total formula weight | 535475.70 |
| Authors | Luqiang, G.,McLellan, J.S. (deposition date: 2025-06-13, release date: 2025-07-30, Last modification date: 2026-03-25) |
| Primary citation | Guo, L.,McFadden, E.,Slough, M.M.,Stone, E.T.,Berrigan, J.,Mittler, E.,Hatzakis, K.,Hinkley, T.,Kain, H.S.,Ke, Z.,Warner, N.L.,Erasmus, J.H.,Chandran, K.,McLellan, J.S. High-resolution in situ structures of hantavirus glycoprotein tetramers. Cell, 2026 Cited by PubMed Abstract: New World hantaviruses cause severe infections in humans. Previous structural studies have advanced our understanding of hantavirus glycoprotein architecture; however, the lack of high-resolution structures of the glycoprotein tetramer and its lattice organization has limited mechanistic insights into viral assembly and entry. Here, we leveraged a virus-like particle (VLP) system to establish a cryo-electron microscopy workflow for lattice-forming viral glycoproteins. This enabled the determination of a 2.35 Å resolution structure of the membrane-embedded Andes virus (ANDV) glycoprotein tetramer as well as the structures of dimers of tetramers and a complex with antibody ADI-65534. These structures reveal previously uncharacterized features of glycoprotein organization, stability, and pH sensing. The immunization of mice with self-amplifying replicon RNA (repRNA) encoding ANDV-VLPs elicited high levels of glycoprotein-binding antibodies but equivalent titers of neutralizing antibodies compared with the repRNA-encoded native ANDV glycoprotein complex. These findings advance our understanding of hantavirus glycoprotein assemblies, laying a foundation for structure-based vaccine design. PubMed: 41763200DOI: 10.1016/j.cell.2026.01.030 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.35 Å) |
Structure validation
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