9OJ3

Crystal structure of the SpyTag003-SpyCatcher003 complex

Summary for 9OJ3

• Entry DOI: 10.2210/pdb9oj3/pdb
• Descriptor: Fibronectin binding protein, ARG-GLY-VAL-PRO-HIS-ILE-VAL-MET-VAL-ASP-ALA-TYR-LYS-ARG-TYR, 6-tungstotellurate(VI), ... (5 entities in total)
• Functional Keywords: complex, peptide binding protein
• Biological source: Streptococcus pyogenes; More
• Total number of polymer chains: 4
• Total formula weight: 24659.01

Authors

Ralston, C.Y. (deposition date: 2025-05-06, release date: 2025-06-25, Last modification date: 2025-07-16)

Primary citation

Ralston, C.Y.,Gupta, S.,Del Mundo, J.T.,Soe, A.C.,Russell, B.,Rad, B.,Tyler, J.,Paul, S.,Kahan, D.N.,Kristensen, L.G.,Subramanian, S.,Kidd, S.,Burnett, K.,Sankaran, B.,Classen, S.,Prigozhin, D.M.,Taylor, J.R.,Dickert, J.M.,Royal, K.B.,Rozales, A.,Ortega, S.L.,Allaire, M.,Nix, J.C.,Hura, G.L.,Holton, J.M.,Hammel, M.,Adams, P.D.
ALS-ENABLE: creating synergy and opportunity at the Advanced Light Source synchrotron structural biology beamlines.
J.Synchrotron Radiat., 32:1059-1067, 2025

PubMed Abstract: ALS-ENABLE is an integrated NIH P30 resource at the Advanced Light Source synchrotron at Lawrence Berkeley National Laboratory in Berkeley, California, USA. The resource provides a single portal to the combined mature structural biology technologies of macromolecular crystallography, small-angle X-ray scattering and X-ray footprinting mass spectrometry, and includes beamlines 2.0.1, 3.3.1, 4.2.2, 5.0.1, 5.0.2, 5.0.3, 8.2.1, 8.2.2, 8.3.1 and 12.3.1. This paper describes the organizational structure and the technologies of ALS-ENABLE. A case study showcasing the main technologies of the resource applied to the characterization of the SpyCatcher-SpyTag protein system is presented.

PubMed: 40531663
DOI: 10.1107/S1600577525004205

Experimental method

X-RAY DIFFRACTION (1.43 Å)