9OCL

PV2-10D2 Complex

Summary for 9OCL

• Entry DOI: 10.2210/pdb9ocl/pdb
• EMDB information: 70318
• Descriptor: Capsid protein VP1, Capsid protein VP2, Capsid protein VP3, ... (7 entities in total)
• Functional Keywords: virus, antibody, complex
• Biological source: Poliovirus 2; More
• Total number of polymer chains: 6
• Total formula weight: 122905.91

Authors

Waddey, B.T.,Hafenstein, S.L. (deposition date: 2025-04-24, release date: 2025-12-17, Last modification date: 2026-01-14)

Primary citation

Waddey, B.T.,Charnesky, A.J.,Faust, J.E.,DiNunno, N.M.,Puligedda, R.D.,Cho, S.H.,Bator, C.M.,Dong, S.D.,Mahmood, K.,Chumakov, K.M.,Dessain, S.K.,Hafenstein, S.L.
Neutralizing human monoclonal antibodies to poliovirus map to the receptor binding site.
Nat Commun, 2026

PubMed Abstract: Poliovirus remains a serious threat to human health. Complete eradication of wild-type poliovirus has not yet succeeded, making the development of successful antivirals critical. Microneutralization assays against all three poliovirus serotypes identified a panel of human monoclonal IgGs, which are either serotype-specific or cross-neutralizing. Here, through cryoEM single particle analysis, we solved high resolution structures of four distinct poliovirus-FAb complexes. These antibodies bind to capsids at the circular depression (canyon) surrounding the icosahedral five-fold symmetry axis, which is also the binding site of the poliovirus receptor (PVR). Analysis of these structures confirms overlap of FAb contacts on the viral capsid with those of PVR. For three of the FAbs, the capsid residues are identified that dictate serotype-specific recognition. Contacts for the cross-neutralizing mAb 10D2 are located deep in the capsid canyon. These structural analyses indicate that antibody competition with the receptor likely leads to neutralization of virus particles and inhibition of poliovirus entry into host cells. Thus, the human IgGs studied here may facilitate development of therapeutics for the ongoing efforts in global eradication of poliovirus.

PubMed: 41484135
DOI: 10.1038/s41467-025-68226-x

Experimental method

ELECTRON MICROSCOPY (3.65 Å)