9NUV
SsoPfMCM:DNA class 1c from DNA 2
Summary for 9NUV
| Entry DOI | 10.2210/pdb9nuv/pdb |
| Related | 9NUH 9NUI 9NUJ 9NUK 9NUL 9NUM 9NUN 9NUO 9NUP 9NUQ 9NUR 9NUS 9NUT 9NUU 9NUW 9NUX 9NUY |
| EMDB information | 49820 |
| Descriptor | Minichromosome maintenance protein MCM, Cell division control protein 21,DNA helicase, DNA (60-MER), ADENOSINE-5'-DIPHOSPHATE, ... (6 entities in total) |
| Functional Keywords | dna replication, helicase, mcm, replication |
| Biological source | Saccharolobus solfataricus P2 More |
| Total number of polymer chains | 8 |
| Total formula weight | 456020.14 |
| Authors | Enemark, E.J.,Rasouli, S.,Myasnikov, A. (deposition date: 2025-03-19, release date: 2026-04-01, Last modification date: 2026-06-17) |
| Primary citation | Rasouli, S.,Myasnikov, A.,Enemark, E.J. Archaeal and eukaryotic MCM rings sequentially melt DNA for replication initiation. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: DNA replication initiation requires local melting of fully base-paired DNA for a helicase to gain a foothold and initiate processive DNA unwinding. In eukaryotes and archaea, the helicase engine is the hexameric ring minichromosome maintenance (MCM) complex. In eukaryotes, a defined biochemical sequence assembles two Cdc45-MCM-GINS (CMG) complexes that provide limited DNA unwinding as the species that immediately precedes extensive unwinding. A prior structure revealed how MCM subunits interact with this form of DNA, but the atomic progression from undistorted DNA to this melted DNA species is unknown. Here, we present a sequential DNA melting mechanism determined by snapshots of an archaeal MCM ring with DNA in varying degrees of melting. In this mechanism, successive ATP-binding at MCM ATPase sites drives sequential discrete DNA melting steps mediated a specific MCM aromatic residue. Analysis of eukaryotic structures shows loaded MCM rings principally adopt only two molecular arrangements at the ATPase: one that does not melt DNA and one tuned to melt DNA with equivalent aromatic residues, indicating a universal sequential mechanism melts DNA in archaea and eukaryotes for replication initiation. PubMed: 41916983DOI: 10.1038/s41467-026-70961-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.51 Å) |
Structure validation
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