9NO1

Cryo-ET map of the VZV capsid vertex (5-fold axis).

This is a non-PDB format compatible entry.

Summary for 9NO1

• Entry DOI: 10.2210/pdb9no1/pdb
• EMDB information: 49591
• Descriptor: ORF40, Small capsomere-interacting protein, ORF20, ... (7 entities in total)
• Functional Keywords: varicella-zoster virus, capsid, cryo-et, cryo-fib, virus
• Biological source: Human alphaherpesvirus 3 (Varicella-zoster virus); More
• Total number of polymer chains: 24
• Total formula weight: 2296222.43

Authors

Oliver, S.L.,Chen, M. (deposition date: 2025-03-07, release date: 2025-07-30, Last modification date: 2026-04-15)

Primary citation

Oliver, S.L.,Chen, M.,Engel, L.,Hecksel, C.W.,Zhou, X.,Schmid, M.F.,Arvin, A.M.,Chiu, W.
Cryogenic electron tomography reveals herpesvirus capsid assembly intermediates inside the cell nucleus.
Nat Commun, 17:-, 2026

PubMed Abstract: Herpesviruses encapsulate their double-stranded DNA (dsDNA) genomes within an icosahedral nucleocapsid formed in the infected cell nucleus. Four biochemically purified nucleocapsids have been characterized, but their roles in herpesvirus replication remain controversial. The status of the capsid vertex-specific component (CVSC), essential for capsid stability and dsDNA packaging and retention, is also unclear. By integrating cryogenic focused ion beam milling with electron tomography and subtomogram averaging, we derive atomic models for all protein components, including the CVSC, across different herpesvirus capsid types within infected cell nuclei. Focused classification of pentonal vertex densities reveal differences in CVSC occupancy between genome-filled capsids and capsids lacking dsDNA, highlight structural heterogeneity and providing insight into distinct capsid assembly stages in situ. These intra-nuclear findings redefine the maturation model of herpesvirus capsid assembly, advancing the understanding of herpesvirus replication, and demonstrate the effectiveness of in situ electron imaging by studying virus assembly within host cells.

PubMed: 41741463
DOI: 10.1038/s41467-026-69811-4

Experimental method

ELECTRON MICROSCOPY (8.3 Å)