9N9O

Cryo-EM structure of the dCas12f-gRNA-dsDNA complex (full R-Loop)

Summary for 9N9O

Entry DOI	10.2210/pdb9n9o/pdb
EMDB information	49174
Descriptor	Nuclease-deactivated Cas12f, DNA non-template strand, DNA template strand, ... (4 entities in total)
Functional Keywords	dcas12f, full r-loop, rna binding protein, rna binding protein-dna-rna complex, rna binding protein/dna/rna
Biological source	Flagellimonas taeanensis More
Total number of polymer chains	5
Total formula weight	152730.03
Authors	Chang, L.,Sternberg, S.H.,Xiao, R.,Hoffmann, F.T.,Wiegand, T.,Xie, D. (deposition date: 2025-02-11, release date: 2025-12-24, Last modification date: 2026-03-04)
Primary citation	Renjian, X.,Hoffmann, F.T.,Xie, D.,Wiegand, T.,Palmieri, A.I.,Sternberg, S.H.,Leifu, C. Structural basis of RNA-guided transcription by a dCas12f-SigmaE-RNAP complex Nature, 2026 Cited by DOI: 10.1038/s41586-026-10178-3 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.42 Å)

Structure validation