9N2C
Impacts of ribosomal RNA sequence variation on gene expression and phenotype: Cryo-EM structure of the rrsH ribosome (HBB-70S)
This is a non-PDB format compatible entry.
Summary for 9N2C
| Entry DOI | 10.2210/pdb9n2c/pdb |
| EMDB information | 48831 |
| Descriptor | 16S ribosomal RNA (rRNA) from the rrnH operon, 30S ribosomal protein S10, Small ribosomal subunit protein uS11, ... (61 entities in total) |
| Functional Keywords | translation, transcription, rdna, rrna, cryo-em, ribosome |
| Biological source | Escherichia coli K-12 More |
| Total number of polymer chains | 54 |
| Total formula weight | 2196231.43 |
| Authors | Welfer, G.A.,Brady, R.A.,Natchiar, S.K.,Watson, Z.L.,Rundlet, E.J.,Alejo, J.L.,Singh, A.P.,Mishra, N.K.,Altman, R.B.,Blanchard, S.C. (deposition date: 2025-01-28, release date: 2025-03-19) |
| Primary citation | Welfer, G.A.,Brady, R.A.,Natchiar, S.K.,Watson, Z.L.,Rundlet, E.J.,Alejo, J.L.,Singh, A.P.,Mishra, N.K.,Altman, R.B.,Blanchard, S.C. Impacts of ribosomal RNA sequence variation on gene expression and phenotype. Philos.Trans.R.Soc.Lond.B Biol.Sci., 380:20230379-20230379, 2025 Cited by PubMed Abstract: Since the framing of the Central Dogma, it has been speculated that physically distinct ribosomes within cells may influence gene expression and cellular physiology. While heterogeneity in ribosome composition has been reported in bacteria, protozoans, fungi, zebrafish, mice and humans, its functional implications remain actively debated. Here, we review recent evidence demonstrating that expression of conserved variant ribosomal DNA (rDNA) alleles in bacteria, mice and humans renders their actively translating ribosome pool intrinsically heterogeneous at the level of ribosomal RNA (rRNA). In this context, we discuss reports that nutrient limitation-induced stress in leads to changes in variant rRNA allele expression, programmatically altering transcription and cellular phenotype. We highlight that cells expressing ribosomes from distinct operons exhibit distinct drug sensitivities, which can be recapitulated and potentially rationalized by subtle perturbations in ribosome structure or in their dynamic properties. Finally, we discuss evidence that differential expression of variant rDNA alleles results in different populations of ribosome subtypes within mammalian tissues. These findings motivate further research into the impacts of rRNA heterogeneities on ribosomal function and predict that strategies targeting distinct ribosome subtypes may hold therapeutic potential.This article is part of the discussion meeting issue 'Ribosome diversity and its impact on protein synthesis, development and disease'. PubMed: 40045785DOI: 10.1098/rstb.2023.0379 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.4 Å) |
Structure validation
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