9MXM
EatA catalytic domain from Mycobacterium abscessus
Summary for 9MXM
| Entry DOI | 10.2210/pdb9mxm/pdb |
| Descriptor | EatA, POTASSIUM ION, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | arabinogalactan, type vii secretion, gh183, beta-propeller, toxin, hydrolase |
| Biological source | Mycobacteroides abscessus subsp. massiliense |
| Total number of polymer chains | 2 |
| Total formula weight | 72529.47 |
| Authors | Benedict, S.T.,Moynihan, P.J. (deposition date: 2025-01-20, release date: 2026-06-24, Last modification date: 2026-08-19) |
| Primary citation | Benedict, S.T.,Bowran, K.,Lee, E.K.E.,Reyre, J.L.,Ahmad, H.,Franklin, A.,Mietrach, N.A.,Han, C.R.,Chan, J.M.,Layton, A.J.,Severi, E.,Boardman, E.R.,Anochshenko, K.,Goudge, G.,Caulton, S.G.,Lowary, T.L.,Lovering, A.L.,Banzhaf, M.,Lowe, E.C.,Palmer, T.,Moynihan, P.J. A Type VII-secreted toxin enables inter-mycobacterial competition. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: Most bacteria have evolved mechanisms to compete with other bacteria, often through the specialised secretion of proteinaceous toxins. However, mycobacteria have not previously been reported to engage in this form of competition. The thick and unusual mycobacterial cell wall, comprised of peptidoglycan, arabinogalactan and mycolic acids, is generally thought to be highly protective to these bacteria. Here, we show that some mycobacteria can use endo-D-arabinanases of the GH183 family for inter-bacterial competition. These microorganisms secrete an endo-D-arabinanase effector via the type VII secretion system (T7SS) that cleaves the arabinogalactan layer of the mycobacterial cell envelope. We describe the molecular basis for this activity using structural biology and biochemistry, and identify a protein family that protects the bacterium from the activity of this toxin. The widespread presence of genes potentially encoding similar T7SS-secreted toxins in the Mycobacteriales suggests extensive inter-mycobacterial competition. PubMed: 42336839DOI: 10.1038/s41467-026-74429-7 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.06 Å) |
Structure validation
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