9MUD
Cryo-EM structure of CRISPR-associated cA4 bound Cat1 Pentagonal filament assembly
Summary for 9MUD
| Entry DOI | 10.2210/pdb9mud/pdb |
| EMDB information | 48629 |
| Descriptor | RNA (5'-R(P*AP*AP*AP*A)-3'), Cat1 (CRISPR associated TIR 1) pentagonal filament (2 entities in total) |
| Functional Keywords | crispr, antiphage defense, adaptive immunity, filament, carf, tir, ca4, antiviral protein |
| Biological source | bacterium More |
| Total number of polymer chains | 45 |
| Total formula weight | 923595.75 |
| Authors | Majumder, P.,Patel, D.J. (deposition date: 2025-01-13, release date: 2025-04-16, Last modification date: 2025-04-23) |
| Primary citation | Baca, C.F.,Majumder, P.,Hickling, J.H.,Patel, D.J.,Marraffini, L.A. Cat1 forms filament networks to degrade NAD + during the type III CRISPR-Cas antiviral response. Science, :eadv9045-eadv9045, 2025 Cited by PubMed Abstract: Type III CRISPR-Cas systems defend against viral infection in prokaryotes using an RNA-guided complex that recognizes foreign transcripts and synthesizes cyclic oligo-adenylate (cOA) messengers to activate CARF immune effectors. Here we investigated a protein containing a CARF domain fused Toll/interleukin-1 receptor (TIR) domain, Cat1. We found that Cat1 provides immunity by cleaving and depleting NAD molecules from the infected host, inducing a growth arrest that prevents viral propagation. Cat1 forms dimers that stack upon each other to generate long filaments that are maintained by bound cOA ligands, with stacked TIR domains forming the NAD cleavage catalytic sites. Further, Cat1 filaments assemble into unique trigonal and pentagonal networks that enhance NAD degradation. Cat1 presents an unprecedented chemistry and higher-order protein assembly for the CRISPR-Cas response. PubMed: 40208959DOI: 10.1126/science.adv9045 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.4 Å) |
Structure validation
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