9MOG
Structure of the distal part of the bacteriophage T4 tail
This is a non-PDB format compatible entry.
Summary for 9MOG
| Entry DOI | 10.2210/pdb9mog/pdb |
| EMDB information | 48459 |
| Descriptor | gp12, short tail fiber protein, gp5.4, tip of the central spike, gp19, tail tube protein, ... (23 entities in total) |
| Functional Keywords | bacteriophage t4, contractile tail, baseplate, viral protein |
| Biological source | Escherichia phage T4 More |
| Total number of polymer chains | 220 |
| Total formula weight | 9609007.02 |
| Authors | Fokine, A.,Zhu, J.,Klose, T.,Vago, F.,Arnaud, C.,Wang, Z.,Khare, B.,Rossmann, M.G.,Chen, Z.,Sun, L.,Fang, Q.,Kuhn, R.,Rao, V.B. (deposition date: 2024-12-26, release date: 2026-01-28, Last modification date: 2026-08-12) |
| Primary citation | Fokine, A.,Zhu, J.,Klose, T.,Vago, F.,Arnaud, C.A.,Wang, Z.,Khare, B.,Rossmann, M.G.,Chen, Z.,Sun, L.,Fang, Q.,Kuhn, R.J.,Rao, V.B. In situ structures of the portal-neck-tail complex of bacteriophage T4 inform a viral genome positioning mechanism. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: The post-genome packaging mechanisms that govern the assembly of an infectious virion are poorly understood in bacteriophages and other viruses. Here, our near-atomic resolution cryo-EM structural analyses uncovered an assembly- and conformation-driven genome positioning mechanism in the tailed bacteriophage T4. We show that following headful packaging, which generates a pressurized head, a global conformational change occurs in the portal structure, probably triggering packaging termination and ejection of the packaging motor. Our high-resolution structures of the neck of the virion further show that the neck undergoes conformational changes upon docking of a pre-assembled tail onto the sealed neck, which then opens a genome-gate. Driven by the pressure of the packaged DNA, the genome travels through open neck channels, binds and compresses the resident tape-measure protein, and halts at the bottom of the second topmost disk of the tail tube. Pressure-suspended within the virion's innermost tunnel and secured by a baseplate plug, the genome remains poised to flow through the tunnel into a host cell upon receiving the host receptor recognition signal. PubMed: 41720772DOI: 10.1038/s41467-026-69106-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.6 Å) |
Structure validation
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