9MKN

Structure of the Respiratory Syncytial Virus Fusion Protein Bound to Human Antibodies RSV_2245 and RSV_3301

Summary for 9MKN

• Entry DOI: 10.2210/pdb9mkn/pdb
• EMDB information: 48331
• Descriptor: Fusion glycoprotein F2, Fusion glycoprotein F1, 2245 Fab Heavy Chain, ... (6 entities in total)
• Functional Keywords: fusion protein, trimer, antibody, complex, antiviral protein, viral protein-immune system complex, viral protein/immune system
• Biological source: Respiratory syncytial virus; More
• Total number of polymer chains: 18
• Total formula weight: 434849.69

Authors

Johnson, N.V.,McLellan, J.S. (deposition date: 2024-12-17, release date: 2025-10-29, Last modification date: 2025-12-24)

Primary citation

Wasdin, P.T.,Johnson, N.V.,Janke, A.K.,Held, S.,Marinov, T.M.,Jordaan, G.,Gillespie, R.A.,Vandenabeele, L.,Pantouli, F.,Powers, O.C.,Vukovich, M.J.,Holt, C.M.,Kim, J.,Hansman, G.,Logue, J.,Chu, H.Y.,Andrews, S.F.,Kanekiyo, M.,Sautto, G.A.,Ross, T.M.,Sheward, D.J.,McLellan, J.S.,Abu-Shmais, A.A.,Georgiev, I.S.
Generation of antigen-specific paired-chain antibodies using large language models.
Cell, 188:7206-, 2025

PubMed Abstract: The traditional process of antibody discovery is limited by inefficiency, high costs, and low success rates. Recent approaches employing artificial intelligence (AI) have been developed to optimize existing antibodies and generate antibody sequences in a target-agnostic manner. In this work, we present MAGE (monoclonal antibody generator), a sequence-based protein language model (PLM) fine-tuned for the task of generating paired human variable heavy- and light-chain antibody sequences against targets of interest. We show that MAGE can generate novel and diverse antibody sequences with experimentally validated binding specificity against SARS-CoV-2, an emerging avian influenza H5N1, and respiratory syncytial virus A (RSV-A). MAGE represents a first-in-class model capable of designing human antibodies against multiple targets with no starting template.

PubMed: 41192421
DOI: 10.1016/j.cell.2025.10.006

Experimental method

ELECTRON MICROSCOPY (3.4 Å)