9MFA
Backbone alpha-Methylation in the Villin Headpiece Miniprotein: HP35 with Aib at Position 30
Summary for 9MFA
Entry DOI | 10.2210/pdb9mfa/pdb |
NMR Information | BMRB: 31223 |
Descriptor | Villin-1 (1 entity in total) |
Functional Keywords | miniprotein, heterogeneous backbone, proteomimetic, structural protein |
Biological source | Gallus gallus (chicken) |
Total number of polymer chains | 1 |
Total formula weight | 4000.60 |
Authors | Harmon, T.W.,Lin, Y.,Sutton, R.T.,Osborne, S.,Horne, W.S. (deposition date: 2024-12-09, release date: 2025-01-22, Last modification date: 2025-03-26) |
Primary citation | Harmon, T.W.,Lin, Y.,Sutton, R.T.,Osborne, S.W.J.,Seth Horne, W. Interplay between C alpha Methylation and C alpha Stereochemistry in the Folding Energetics of a Helix-Rich Miniprotein. Chembiochem, 26:e202401022-e202401022, 2025 Cited by PubMed Abstract: The α-helix is an abundant and functionally important element of protein secondary structure, which has motivated intensive efforts toward chemical strategies to stabilize helical folds. One such method is the incorporation of non-canonical backbone composition through an additional methyl substituent at the Cα atom. Examples of monomers include the achiral 2-aminoisobutyric acid (Aib) with geminal dimethyl substitution and chiral analogues with one methyl and one non-methyl substituent. While Aib and chiral Cα-Me residues are both established helix promoting moieties, their comparative ability in this regard has not been quantitatively investigated. Addressing this gap would help to inform the use of these building blocks in the construction of peptide and protein mimetics as well as provide fundamental insights into consequences of backbone methylation on folding. Here, we report a quantitative comparison of the impacts of Aib and chiral αMe residues on the high-resolution folded structure and folding thermodynamics of a small helical protein. These results reveal a synergistic stabilizing effect arising from the presence of Cα methylation in conjunction with a Cα stereocenter. PubMed: 39791987DOI: 10.1002/cbic.202401022 PDB entries with the same primary citation |
Experimental method | SOLUTION NMR |
Structure validation
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