9MB7

Cryo-EM structure of eSaCas9-NNG-guide RNA-mismatched target DNA complex

Summary for 9MB7

Entry DOI	10.2210/pdb9mb7/pdb
EMDB information	63768
Descriptor	target DNA strand, non-target DNA strand, Ubiquitin-like protein SMT3,CRISPR-associated endonuclease Cas9, ... (5 entities in total)
Functional Keywords	crispr-cas9, genome engineering, hydrolase-rna-dna complex, dna binding protein
Biological source	Staphylococcus aureus More
Total number of polymer chains	4
Total formula weight	196173.68
Authors	Omura, S.N.,Nakagawa, R.,Yamashita, K.,Nishimasu, H.,Nureki, O. (deposition date: 2025-03-15, release date: 2026-03-18, Last modification date: 2026-04-15)
Primary citation	Nakagawa, R.,Omura, S.N.,Kajimoto, S.,Okazaki, S.,Ishiguro, S.,Mori, H.,Onishi, K.,Kashiwakura, Y.,Hiramoto, T.,Hirano, H.,Yamashita, K.,Jividen, K.,Tsai, S.Q.,Yachie, N.,Ohmori, T.,Nishimasu, H.,Nureki, O. Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation. Nat Commun, 2026 Cited by DOI: 10.1038/s41467-026-71626-2 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.88 Å)

Structure validation