9MB7

Cryo-EM structure of eSaCas9-NNG-guide RNA-mismatched target DNA complex

Summary for 9MB7

• Entry DOI: 10.2210/pdb9mb7/pdb
• EMDB information: 63768
• Descriptor: target DNA strand, non-target DNA strand, Ubiquitin-like protein SMT3,CRISPR-associated endonuclease Cas9, ... (5 entities in total)
• Functional Keywords: crispr-cas9, genome engineering, hydrolase-rna-dna complex, dna binding protein
• Biological source: Staphylococcus aureus; More
• Total number of polymer chains: 4
• Total formula weight: 196173.68

Authors

Nakagawa, R.,Omura, S.N.,Yamashita, K.,Nishimasu, H.,Nureki, O. (deposition date: 2025-03-15, release date: 2026-03-18)

• Primary citation: Nakagawa, R.,Omura, S.N.,Kajimoto, S.,Okazaki, S.,Ishiguro, S.,Mori, H.,Kashiwakura, Y.,Hiramoto, T.,Hirano, H.,Yamashita, K.,Jividen, K.,Tsai, S.Q.,Yachie, N.,Ohmori, T.,Nishimasu, H.,Nureki, O.; Molecular engineering and structural mechanism of the compact CRISPR-Cas9 nuclease; To Be Published,

Experimental method

ELECTRON MICROSCOPY (2.88 Å)