9M3J
structure of bundle-shaped PBS with both long rod and (ApcA2B3ApcD) trimer
This is a non-PDB format compatible entry.
Summary for 9M3J
| Entry DOI | 10.2210/pdb9m3j/pdb |
| Related | 9K9W |
| EMDB information | 62201 63604 |
| Descriptor | Allophycocyanin alpha subunit, Allophycocyanin beta subunit, Allophycocyanin-B, ... (6 entities in total) |
| Functional Keywords | phycobilisome, light-harvesting complex, photosynthesis |
| Biological source | Gloeobacter violaceus PCC 7421 More |
| Total number of polymer chains | 104 |
| Total formula weight | 2014058.38 |
| Authors | Ma, J.,Sui, S.-F. (deposition date: 2025-03-02, release date: 2025-12-24, Last modification date: 2026-01-07) |
| Primary citation | Ma, J.,You, X.,Sun, S.,Sui, S.F. Light-induced structural adaptation of the bundle-shaped phycobilisome from thylakoid-lacking cyanobacterium Gloeobacter violaceus. Nat Commun, 16:5956-5956, 2025 Cited by PubMed Abstract: Gloeobacter diverged from other lineages early in cyanobacterial evolution, preferentially growing under low light intensity conditions. Among cyanobacteria, G. violaceus exhibits unique features, including lack of a thylakoid membrane and bundle-shaped antenna phycobilisomes (PBSs), densely packed and well-organized on the plasma membrane. However, without high-resolution structures, it has remained unclear how G. violaceus PBSs assemble into a bundle-shaped configuration. Here we solve the cryo-EM structures of PBSs from G. violaceus cells cultured under low (Sr-PBS) or moderate (Lr-PBS) light intensity. These structures reveal two unique linker proteins, L and L, that play a key role in the PBS architecture. Analysis of the bilin arrangement indicates that the bundle-shaped structure allows efficient energy transfer among rods. Moreover, comparison between Lr-PBS and Sr-PBS uncovers a distinct mode of adaption to increased light intensity wherein the ApcA-ApcB-ApcD layer can be blocked from binding to the core by altering structural elements exclusively found in the G. violaceus L. This study illustrates previously unrecognized mechanisms of assembly and adaptation to varying light intensity in the bundle-shaped PBS of G. violaceus. PubMed: 40593595DOI: 10.1038/s41467-025-60673-w PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.37 Å) |
Structure validation
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