9LKB
Cryo-EM structure of the receptor of PL45-Olfr110-Gs complex
This is a non-PDB format compatible entry.
Summary for 9LKB
| Entry DOI | 10.2210/pdb9lkb/pdb |
| EMDB information | 63174 |
| Descriptor | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Olfactory receptor, ... (4 entities in total) |
| Functional Keywords | olfr110, receptor, membrane protein |
| Biological source | synthetic construct More |
| Total number of polymer chains | 3 |
| Total formula weight | 115409.06 |
| Authors | Rong, N.K.,Zhang, M.H.,Yang, F.,Sun, J.P. (deposition date: 2025-01-16, release date: 2026-01-21, Last modification date: 2026-02-18) |
| Primary citation | Han, X.,Zhang, M.H.,Rong, N.K.,Zhu, K.K.,Pei, Y.,Ge, X.Y.,Liu, Q.,Lu, H.,Liu, J.,Zhang, L.J.,Bao, H.Q.,Pi, J.H.,Fan, L.,Fu, Y.,Shao, X.H.,Yan, W.Q.,Gao, X.H.,Zhang, M.Y.,Guo, L.L.,Lu, Y.,Ning, S.L.,Zhang, H.,Xu, Y.F.,Feng, X.Y.,Cheng, J.,Xia, M.,Li, Q.,Yu, X.,Yang, F.,Sun, J.P. Mechanistic insights into fatty acid odor detection mediated by class II olfactory receptors. Cell, 2026 Cited by PubMed Abstract: Smell is one of the fundamental senses mediated by thousands of odorant receptors (ORs). How hydrophobic and volatile odor molecules are recognized by class II ORs remains elusive. Here, we present cryo-electron microscopy (cryo-EM) structures of class II OR Olfr110 complexed with the unsaturated fatty acid metabolite (UFAM) PL45, Gs, and cons-OR5. The structural study revealed an unusually large hydrophobic pocket accommodating PL45 and the endogenous agonist 12(S)-hydroxyeicosapentaenoic acid (12(S)-HEPE). This pocket is decorated with polar residues and aromatic residue arrays, constituting polar networks and π-π interactions with the natural agonist PL45, respectively. Conserved motifs in the type II OR5 subfamily responsible for ligand recognition are characterized. The inward movement of extracellular loop 3 (ECL3) and an unconventional activation mechanism underlie Olfr110 activation. At the G protein interface, Olfr110 displays common and unique interactions. Overall, we revealed the structural basis of odor recognition and the activation mechanism of class II ORs, which may facilitate drug development targeting these receptors. PubMed: 41570821DOI: 10.1016/j.cell.2025.12.018 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.7 Å) |
Structure validation
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