9L8T

The apo structure (State 1) of African Swine Fever Virus DNA polymerase

Summary for 9L8T

• Entry DOI: 10.2210/pdb9l8t/pdb
• EMDB information: 62890
• Descriptor: DNA polymerase (1 entity in total)
• Functional Keywords: asfv, dna polymerase, virus, dna binding protein
• Biological source: African swine fever virus
• Total number of polymer chains: 1
• Total formula weight: 115258.23

Authors

Hu, Z.,Yan, R. (deposition date: 2024-12-28, release date: 2025-12-31, Last modification date: 2026-01-28)

Primary citation

Hu, Z.,SunKang, Y.,Liu, Z.,Huang, L.,Qi, W.,Yan, R.
Structural basis for DNA replication by the African swine fever virus polymerase.
Iscience, 28:114155-114155, 2025

PubMed Abstract: African swine fever virus (ASFV) devastates swine herds and lacks widely available antivirals. We show that the ASFV DNA polymerase beta (Pol β; gene ) localizes to viral replication factories and is required for viral replication. Cryo-electron microscopy structures of Pol β in apo and double-stranded DNA (dsDNA)-bound states reveal pronounced conformational flexibility that enables transitions between functional states. Binding to dsDNA promotes higher-order oligomerization that is essential for catalytic activity, as demonstrated by structure-guided mutagenesis and biochemical assays. These findings define the structural basis of ASFV genome synthesis, highlight the functional significance of Pol β during viral replication, and provide a framework for polymerase-targeted antiviral discovery.

PubMed: 41438038
DOI: 10.1016/j.isci.2025.114155

Experimental method

ELECTRON MICROSCOPY (2.92 Å)