9L2W

Crystal structure of the C-terminal of viral responsive protein 15 (PmVRP15) from black tiger shrimp Penaeus monodon

Summary for 9L2W

• Entry DOI: 10.2210/pdb9l2w/pdb
• Descriptor: Viral responsive protein (2 entities in total)
• Functional Keywords: shrimp protein, viral responsive protein, white spot syndrome virus partner, unknown function
• Biological source: Penaeus monodon (black tiger shrimp)
• Total number of polymer chains: 3
• Total formula weight: 21156.61

Authors

Laohawutthichai, P.,Kim, S.-Y.,Chek, M.F.,Krusong, K.,Hakoshima, T. (deposition date: 2024-12-17, release date: 2025-10-22)

Primary citation

Laohawutthichai, P.,Kim, S.Y.,Chek, M.F.,Jatuyosporn, T.,Tassanakajon, A.,Krusong, K.,Hakoshima, T.
Structure of the C-terminal of Viral Responsive Protein 15 (VRP15): A Key Protein During White Spot Syndrome Virus (WSSV) Infection.
J.Mol.Biol., 437:169329-169329, 2025

PubMed Abstract: White spot syndrome virus (WSSV) is a major serious threat to black tiger shrimp farming. WSSV infection induces a host protein, viral responsive protein 15 (VRP15), for viral assembly and nuclear egress. Here, we showed that the C-terminal tail of VRP15 (VRP15-C) interacts directly with the viral nucleocapsid tegument protein WSV399. The crystal structure of VRP15-C was determined at 1.5 Å. The structure showed that VRP15-C forms a dimer by forming an α-helix bundle structure and that the dimer further interacts with adjacent dimers to form a tetramer and a higher oligomer by intermolecular helix-helix interactions. The ability to form oligomeric forms may contribute to assembly of viral proteins to form a nucleocapsid of WSSV.

PubMed: 40639753
DOI: 10.1016/j.jmb.2025.169329

Experimental method

X-RAY DIFFRACTION (1.5 Å)