9KPO

Fusobacterium nucleatum ARRPOF RNA dimer conformation 1

Summary for 9KPO

• Entry DOI: 10.2210/pdb9kpo/pdb
• EMDB information: 62489
• Descriptor: RNA (255-MER) (1 entity in total)
• Functional Keywords: cryoem, rna
• Biological source: Fusobacterium nucleatum
• Total number of polymer chains: 2
• Total formula weight: 164358.86

Authors

Tu, Y.F.,Shang, S.T.,Su, Z.M. (deposition date: 2024-11-23, release date: 2025-03-19, Last modification date: 2025-05-14)

Primary citation

Wang, L.,Xie, J.,Gong, T.,Wu, H.,Tu, Y.,Peng, X.,Shang, S.,Jia, X.,Ma, H.,Zou, J.,Xu, S.,Zheng, X.,Zhang, D.,Liu, Y.,Zhang, C.,Luo, Y.,Huang, Z.,Shao, B.,Ying, B.,Cheng, Y.,Guo, Y.,Lai, Y.,Huang, D.,Liu, J.,Wei, Y.,Sun, S.,Zhou, X.,Su, Z.
Cryo-EM reveals mechanisms of natural RNA multivalency.
Science, 388:545-550, 2025

PubMed Abstract: Homo-oligomerization of biological macromolecules leads to functional assemblies that are critical to understanding various cellular processes. However, RNA quaternary structures have rarely been reported. Comparative genomics analysis has identified RNA families containing hundreds of sequences that adopt conserved secondary structures and likely fold into complex three-dimensional structures. In this study, we used cryo-electron microscopy (cryo-EM) to determine structures from four RNA families, including ARRPOF and OLE forming dimers and ROOL and GOLLD forming hexameric, octameric, and dodecameric nanostructures, at 2.6- to 4.6-angstrom resolutions. These homo-oligomeric assemblies reveal a plethora of structural motifs that contribute to RNA multivalency, including kissing-loop, palindromic base-pairing, A-stacking, metal ion coordination, pseudoknot, and minor-groove interactions. These results provide the molecular basis of intermolecular interactions driving RNA multivalency with potential functional relevance.

PubMed: 40080543
DOI: 10.1126/science.adv3451

Experimental method

ELECTRON MICROSCOPY (3.98 Å)