9KO9
Cryo-EM structure of PsCas9-sgRNA binary complex
Summary for 9KO9
| Entry DOI | 10.2210/pdb9ko9/pdb |
| EMDB information | 62471 |
| Descriptor | CRISPR-associated endonuclease Cas9, RNA (131-MER) (2 entities in total) |
| Functional Keywords | crispr-cas9, rna, binary complex, rna binding protein/rna, rna binding protein-rna complex |
| Biological source | Parasutterella secunda More |
| Total number of polymer chains | 2 |
| Total formula weight | 207320.81 |
| Authors | Shen, P.P.,Lu, X.Y.,Huang, J.-W.,Chen, C.-C.,Gut, R.-T. (deposition date: 2024-11-20, release date: 2025-07-30) |
| Primary citation | Shen, P.,Lu, X.,Wang, C.,Huang, J.W.,Min, J.,Luo, J.,Sun, Y.,Chen, C.C.,Guo, R.T. Structural and Mechanistic Investigations of a High-Fidelity Cas9 from Parasutterella secunda Acs Catalysis, 15:10109-10118, 2025 Cited by DOI: 10.1021/acscatal.5c02993PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.7 Å) |
Structure validation
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