9KJB

Crystal structure of Anti-CRISPR AcrIE7

Summary for 9KJB

• Entry DOI: 10.2210/pdb9kjb/pdb
• Descriptor: AcrIE7 (2 entities in total)
• Functional Keywords: acrie7, anti-crispr protein, type i-e crispr cas system, immune system
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 1
• Total formula weight: 13028.71

Authors

Kim, D.Y.,Park, H.H. (deposition date: 2024-11-12, release date: 2025-04-16)

Primary citation

Kim, D.Y.,Lee, S.Y.,Ha, H.J.,Park, H.H.
AcrIE7 inhibits the CRISPR-Cas system by directly binding to the R-loop single-stranded DNA.
Proc.Natl.Acad.Sci.USA, 122:e2423205122-e2423205122, 2025

PubMed Abstract: The CRISPR-Cas system is a well-known adaptive immune system in bacteria, and a prominent mechanism for evading this immunity involves anti-CRISPR (Acr) proteins, which employ various methods to neutralize the CRISPR-Cas system. In this study, using structural and biochemical analyses, we revealed that AcrIE7 binds to the single-stranded DNA in the R-loop formed when Cascade encounters the target DNA, thereby preventing Cas3 from cleaving the DNA. This represents a different inhibition strategy distinct from previously reported Acr mechanisms and offers insights into CRISPR-Cas inhibition.

PubMed: 40178896
DOI: 10.1073/pnas.2423205122

Experimental method

X-RAY DIFFRACTION (2.11 Å)