9KJ9

Anti-CRISPR protein AcrVIA6

Summary for 9KJ9

• Entry DOI: 10.2210/pdb9kj9/pdb
• Descriptor: Anti-CRISPR protein (2 entities in total)
• Functional Keywords: acrvia6, immune system, dna binding protein, structural protein
• Biological source: Rhodobacter capsulatus
• Total number of polymer chains: 1
• Total formula weight: 10599.12

Authors

Han, J.H.,Park, H.H. (deposition date: 2024-11-12, release date: 2025-07-02)

Primary citation

Han, J.H.,Lee, S.Y.,Park, H.H.
AcrVIA6 Is a Monomeric DNA-Binding Protein That Does Not Directly Inhibit Cas13a.
Faseb J., 39:e70753-e70753, 2025

PubMed Abstract: The CRISPR-Cas system is a crucial adaptive immune mechanism in prokaryotes, providing defense against invading genetic elements. Among various CRISPR-Cas systems, the type VI-A system, employing RNA-guided RNase Cas13a, has been extensively studied for its ability to target and degrade single-stranded RNA. Anti-CRISPR (Acr) proteins have evolved as natural inhibitors of these systems, with AcrVIA proteins specifically targeting the Cas13a enzyme. However, there is currently conflicting debate regarding the anti-CRISPR function of AcrVIA6. This study reveals that AcrVIA6 functions as a DNA-binding protein rather than a Cas13a inhibitor, as it does not block RNA cleavage. These findings challenge its role in CRISPR-Cas regulation.

PubMed: 40540289
DOI: 10.1096/fj.202500684R

Experimental method

X-RAY DIFFRACTION (2.39 Å)