9K9L

Cryo-EM structure of the human CENP-A-H4 octasome.

9K9L の概要

• エントリーDOI: 10.2210/pdb9k9l/pdb
• EMDBエントリー: 62193
• 分子名称: Histone H3-like centromeric protein A, Histone H4, Widom601 DNA FW (145-MER), ... (4 entities in total)
• 機能のキーワード: subnucleosome, nuclear protein, dna binding protein-dna complex, dna binding protein, dna binding protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 201442.48

構造登録者

Nozawa, K.,Takizawa, Y.,Kurumizaka, H. (登録日: 2024-10-27, 公開日: 2025-04-30)

主引用文献

Kawasaki, O.,Takizawa, Y.,Kiyokawa, I.,Kurumizaka, H.,Nozawa, K.
Cryo-EM Analysis of a Unique Subnucleosome Containing Centromere-Specific Histone Variant CENP-A.
Genes Cells, 30:e70016-e70016, 2025

PubMed Abstract: In eukaryotes, genomic DNA is stored in the nucleus as nucleosomes, in which a DNA segment is wrapped around a protein octamer consisting of two each of the four histones, H2A, H2B, H3, and H4. The core histones can be replaced by histone variants or altered with covalent modifications, contributing to the regulation of chromosome structure and nuclear activities. The formation of an octameric histone core in nucleosomes is widely accepted. Recently, the H3-H4 octasome, a novel nucleosome-like structure with a histone octamer consisting solely of H3 and H4, has been reported. CENP-A is the centromere-specific histone H3 variant and determines the position of kinetochore assembly during mitosis. CENP-A is a distant H3 variant sharing approximately 50% amino acid sequence with H3. In this study, we found that CENP-A and H4 also formed an octamer without H2A and H2B in vitro. We determined the structure of the CENP-A-H4 octasome at 3.66 Å resolution. In the CENP-A-H4 octasome, an approximately 120-base pair DNA segment was wrapped around the CENP-A-H4 octameric core and displayed the four CENP-A RG-loops, which are the direct binding sites for another centromeric protein, CENP-N.

PubMed: 40129080
DOI: 10.1111/gtc.70016

実験手法

ELECTRON MICROSCOPY (3.66 Å)