9JZO

Crystal structure of PHICD111_20024_EAD.

Summary for 9JZO

• Entry DOI: 10.2210/pdb9jzo/pdb
• Descriptor: Endolysine, ZINC ION (3 entities in total)
• Functional Keywords: antibiotic, endolysin
• Biological source: Clostridium phage phiCD111
• Total number of polymer chains: 2
• Total formula weight: 38374.65

Authors

Hwang, J.S.,Seo, M.D. (deposition date: 2024-10-14, release date: 2025-09-24)

Primary citation

Park, J.,Yoo, S.,An, J.,Cho, Y.,Park, K.,Hwang, J.,Chun, S.I.,Kang, N.J.,Seo, M.D.,Yoon, H.
In vitro characterization of PHICD111_20024_EAD as an engineered endolysin against Clostridioides difficile.
J Glob Antimicrob Resist, 45:1-10, 2025

PubMed Abstract: Clostridioides difficile is a Gram-positive, spore-forming obligate anaerobe that can cause symptoms such as diarrhoea and abdominal cramping and lead to conditions including pseudomembranous colitis. The mainstay treatment of C. difficile infection (CDI) is antibiotics; however, antibiotics-induced gut microbiota dysbiosis poses a potential risk for refractory CDI and increased CDI recurrences. Recently, there has been growing interest in bacteriophages and their lytic enzymes as alternatives to antibiotics. We aimed to develop an engineered endolysin to specifically inactivate C. difficile.

PubMed: 40845944
DOI: 10.1016/j.jgar.2025.08.009

Experimental method

X-RAY DIFFRACTION (1.4 Å)