9JWD

Crystal structure of RNAs A treated with sodium cyanide

Summary for 9JWD

• Entry DOI: 10.2210/pdb9jwd/pdb
• Descriptor: Ribonuclease pancreatic, CYANIDE ION, GLYCEROL, ... (6 entities in total)
• Functional Keywords: hydrolase
• Biological source: Bos taurus (domestic cattle)
• Total number of polymer chains: 1
• Total formula weight: 14028.86

Authors

Ahmad, M.S.,Shah, N. (deposition date: 2024-10-10, release date: 2024-12-04)

Primary citation

Shah, N.,Akbar, Z.,Ahmad, M.S.
Cyanide mediated conformational changes resulted in the displacement of sulfate ion from the active site of bovine pancreatic ribonuclease A.
Biochem.Biophys.Res.Commun., 736:150868-150868, 2024

PubMed Abstract: Ribonuclease A is a major hydrolyzing enzyme involved in the hydrolysis of RNA. The crystals of bovine pancreatic RNase A (bpRNase A) were grown at pH 5.5. The effect of sodium cyanide on bpRNase A was assessed by adding it directly to the crystal containing well. Treating the crystals of bpRNase A with sodium cyanide resulted in the displacement of the sulfate ion from the active site of bpRNase A, while the additional sulfate ion, bound to Ala-4, remained unaffected. The addition of sodium cyanide to bpRNase A crystals did not show change in the secondary structure elements of the enzyme. This study was conducted to check the effect of cyanide on bpRNase A crystals and to displace sulfate ion from its active site.

PubMed: 39490154
DOI: 10.1016/j.bbrc.2024.150868

Experimental method

X-RAY DIFFRACTION (2.22 Å)