9JGO

Structure of Pd ions bound to human heavy chain ferritin nanocage.

Summary for 9JGO

• Entry DOI: 10.2210/pdb9jgo/pdb
• Descriptor: Ferritin heavy chain, N-terminally processed, MAGNESIUM ION, PALLADIUM ION, ... (4 entities in total)
• Functional Keywords: granzyme b; nanovesicles; ferritin; palladium, apoptosis
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 21410.21

Authors

Liu, Y.J.,Zhao, M.,Chen, C.,Hu, X.Y.,Kang, H.L.,Liu, Q.Q.,Huang, X.L. (deposition date: 2024-09-08, release date: 2025-09-10, Last modification date: 2026-03-25)

Primary citation

Hu, X.,Liu, Q.,Kang, H.,Zhao, M.,Zhou, Z.,Li, Y.,Peng, W.,Qi, T.,Liu, R.,Jiao, L.,Zhuang, J.,Liu, Y.,Mann, S.,Huang, X.
Granzyme B-mimetic nanozyme for nanovesicle targeted anticancer applications.
Nat Commun, 17:-, 2026

PubMed Abstract: Cytotoxic T lymphocytes play a crucial role in anti-tumour immunity, with granzyme B (GrB) being a decisive factor in this process. Developing a GrB-based delivery system to mimic T cell-based immunotherapy holds promise but remains challenging. Here, we present an artificial metalloenzyme (nanozyme) with GrB-like protease activity capable of inducing caspase-dependent cell apoptosis. The nanozyme is based on the site-specific binding of Pd(II) ions to recombinant human heavy chain apo-ferritin nanocages to generate a binuclear catalytic centre consisting of two Pd atoms with bridging cysteine ligands, monodentate methionine and histidine residues, and two water molecules. We show that encapsulation of the Pd-ferritin complex within membrane-fused lipid nanoparticles comprising surface-displayed single-chain antibodies affords GrB-mimicking nanozyme-based nanovesicles capable of receptor-mediated delivery of the nanozyme into the cytoplasm of tumour cells and induction of caspase-dependent apoptosis. This study provides valuable insights into the construction of nano-delivery systems with artificial GrB activity and presents a promising therapeutic option for solid tumours.

PubMed: 41587995
DOI: 10.1038/s41467-026-68773-x

Experimental method

X-RAY DIFFRACTION (1.851 Å)