9J9W
A broad-spectrum anti-fungal effector dictates bacterial-fungal interkingdom interactions
Summary for 9J9W
| Entry DOI | 10.2210/pdb9j9w/pdb |
| Descriptor | TsiN, TseN (3 entities in total) |
| Functional Keywords | protein complex, t6ss, toxin effector, dnase activity, toxin |
| Biological source | Paracidovorax citrulli AAC00-1 (Acidovorax citrulli) More |
| Total number of polymer chains | 2 |
| Total formula weight | 34937.80 |
| Authors | |
| Primary citation | Yan, S.,Zou, Y.,Wu, T.,Kan, Y.,Luo, H.,Pei, T.T.,Liang, X.,An, Y.,Meng, P.,Song, Y.,Qin, W.M.,Chen, C.,Dong, T. A broad-spectrum anti-fungal effector dictates bacterial-fungal interkingdom interactions. Plos Pathog., 21:e1013598-e1013598, 2025 Cited by PubMed Abstract: Bacteria-fungi interactions play crucial roles in shaping microbial communities across diverse environmental and host-associated niches. While their antagonism through diffusible metabolites is a well-known ecological phenomenon, delivery of bacterial effectors into the nucleus of fungal cells remains rare, and the mechanisms are poorly understood. Here, we identify and characterize a potent anti-fungal nuclease effector, TseN, delivered by the type VI secretion system (T6SS) of Acidovorax citrulli. TseN possesses a nuclear localization signal and inhibits multiple fungal species, including emerging human pathogens Candida auris and Cryptococcus neoformans. Structural and biochemical analyses show that TseN possesses a unique C-terminal DNase domain that defines a new effector class, with its activity neutralized by a cognate immunity protein TsiN. The delivery of TseN requires the upstream-encoded VgrG5, a chaperone Aave_2128, and PAAR5. Transcriptome profiling of co-cultured bacterial-fungal cells demonstrates that the T6SS attack triggers extensive reprogramming in fungal cells, affecting DNA repair, stress response, and filamentation pathways. These responses not only compromise fungal survival but also modulate fungal drug resistance, as evidenced by the synergistic enhancement of azole efficacy against drug-resistant Candida albicans. Importantly, in vivo experiments confirm that the T6SS, via TseN, can significantly reduce fungal burden on murine skin. Phylogenetic analysis shows TseN homologs are present in a large number of bacterial species. Collectively, our findings highlight a previously underappreciated interkingdom antagonism modulated by a novel effector. The broad-spectrum anti-fungal activities of TseN and its homologs may be explored for therapeutic strategies targeting fungal pathogens in both clinical and environmental settings. PubMed: 41144565DOI: 10.1371/journal.ppat.1013598 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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